Transcriptional inhibition of feline immunodeficiency virus by alpha-amanitin. 2021

Taishi Tanabe, and Yurina Fukuda, and Kazuhiko Kawashima, and Satomi Yamamoto, and Takashige Kashimoto, and Hisaaki Sato
Laboratory of Veterinary Microbiology, School of Veterinary Medicine, Kitasato University, Towada, Aomori 034-8628, Japan.

Alpha-amanitin, one of the amatoxins in egg amanita, has a cyclic peptide structure, and was reported as having antiviral activity against several viruses. We investigated whether α-amanitin has antiviral activity against feline immunodeficiency virus (FIV). FL-4 cells persistently infected with FIV Petaluma were cultured with α-amanitin. Reverse transcriptase (RT) activity in the supernatant of FL-4 cells was significantly inhibited by α-amanitin. In addition, the production of FIV core protein in FL-4 cells was inhibited by α-amanitin when analyzed by western blotting. Furthermore, α-amanitin inhibited the transcription of FIV in real-time RT-PCR. These data suggested that α-amanitin showed anti-FIV activity by inhibiting the RNA transcription level.

UI MeSH Term Description Entries
D002415 Cats The domestic cat, Felis catus, of the carnivore family FELIDAE, comprising over 30 different breeds. The domestic cat is descended primarily from the wild cat of Africa and extreme southwestern Asia. Though probably present in towns in Palestine as long ago as 7000 years, actual domestication occurred in Egypt about 4000 years ago. (From Walker's Mammals of the World, 6th ed, p801) Felis catus,Felis domesticus,Domestic Cats,Felis domestica,Felis sylvestris catus,Cat,Cat, Domestic,Cats, Domestic,Domestic Cat
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000998 Antiviral Agents Agents used in the prophylaxis or therapy of VIRUS DISEASES. Some of the ways they may act include preventing viral replication by inhibiting viral DNA polymerase; binding to specific cell-surface receptors and inhibiting viral penetration or uncoating; inhibiting viral protein synthesis; or blocking late stages of virus assembly. Antiviral,Antiviral Agent,Antiviral Drug,Antivirals,Antiviral Drugs,Agent, Antiviral,Agents, Antiviral,Drug, Antiviral,Drugs, Antiviral
D016089 Immunodeficiency Virus, Feline A species of LENTIVIRUS, subgenus feline lentiviruses (LENTIVIRUSES, FELINE) isolated from cats with a chronic wasting syndrome, presumed to be immune deficiency. There are 3 strains: Petaluma (FIP-P), Oma (FIP-O) and Puma lentivirus (PLV). There is no antigenic relationship between FIV and HIV, nor does FIV grow in human T-cells. FIV,FTLV,Feline Immunodeficiency Virus,Feline T-Lymphotropic Lentivirus,Lentivirus, Puma,Puma lentivirus,Feline T-Lymphotropic Virus,Feline Immunodeficiency Viruses,Feline T Lymphotropic Lentivirus,Feline T Lymphotropic Virus,Lentivirus, Feline T-Lymphotropic,T-Lymphotropic Lentivirus, Feline,T-Lymphotropic Virus, Feline
D053959 Alpha-Amanitin A cyclic octapeptide with a thioether bridge between the cystine and tryptophan. It inhibits RNA POLYMERASE II. Poisoning may require LIVER TRANSPLANTATION. Alpha-Amanitine,Alpha Amanitin,Alpha Amanitine

Related Publications

Taishi Tanabe, and Yurina Fukuda, and Kazuhiko Kawashima, and Satomi Yamamoto, and Takashige Kashimoto, and Hisaaki Sato
May 2012, Viruses,
Taishi Tanabe, and Yurina Fukuda, and Kazuhiko Kawashima, and Satomi Yamamoto, and Takashige Kashimoto, and Hisaaki Sato
October 1970, Nature,
Taishi Tanabe, and Yurina Fukuda, and Kazuhiko Kawashima, and Satomi Yamamoto, and Takashige Kashimoto, and Hisaaki Sato
August 2002, Bioorganic & medicinal chemistry letters,
Taishi Tanabe, and Yurina Fukuda, and Kazuhiko Kawashima, and Satomi Yamamoto, and Takashige Kashimoto, and Hisaaki Sato
April 1996, The veterinary quarterly,
Taishi Tanabe, and Yurina Fukuda, and Kazuhiko Kawashima, and Satomi Yamamoto, and Takashige Kashimoto, and Hisaaki Sato
January 1971, Archiv fur die gesamte Virusforschung,
Taishi Tanabe, and Yurina Fukuda, and Kazuhiko Kawashima, and Satomi Yamamoto, and Takashige Kashimoto, and Hisaaki Sato
February 1970, Journal of virology,
Taishi Tanabe, and Yurina Fukuda, and Kazuhiko Kawashima, and Satomi Yamamoto, and Takashige Kashimoto, and Hisaaki Sato
December 1979, Journal of virology,
Taishi Tanabe, and Yurina Fukuda, and Kazuhiko Kawashima, and Satomi Yamamoto, and Takashige Kashimoto, and Hisaaki Sato
October 1970, Zeitschrift fur Naturforschung. Teil B, Chemie, Biochemie, Biophysik, Biologie und verwandte Gebiete,
Taishi Tanabe, and Yurina Fukuda, and Kazuhiko Kawashima, and Satomi Yamamoto, and Takashige Kashimoto, and Hisaaki Sato
September 2000, The Veterinary clinics of North America. Small animal practice,
Taishi Tanabe, and Yurina Fukuda, and Kazuhiko Kawashima, and Satomi Yamamoto, and Takashige Kashimoto, and Hisaaki Sato
August 1990, Australian veterinary journal,
Copied contents to your clipboard!