BIOMAT Database Logo BIOMAT Database Logo

Adrenomedullin Inhibits Osmotic Water Permeability in Rat Inner Medullary Collecting Ducts. 2020

Fuying Ma, and Guangping Chen, and Eva L Rodriguez, and Janet D Klein, and Jeff M Sands, and Yanhua Wang
Renal Division, Department of Medicine, Emory University, Atlanta, GA 30322, USA.

Adrenomedullin (ADM) is a vasodilator that causes natriuresis and diuresis. However, the direct effect of ADM on osmotic water permeability in the rat inner medullary collecting duct (IMCD) has not been tested. We investigated whether ADM and its ADM receptor components (CRLR, RAMP2, and 3) are expressed in rat inner medulla (IM) and whether ADM regulates osmotic water permeability in isolated perfused rat IMCDs. The mRNAs of ADM, CRLR, and RAMP2 and 3 were detected in rat IM. Abundant protein of CRLR and RAMP3 were also seen but RAMP2 protein level was extremely low. Adding ADM (100 nM) to the bath significantly decreased osmotic water permeability. ADM significantly decreased aquaporin-2 (AQP2) phosphorylation at Serine 256 (pS256) and increased it at Serine 261 (pS261). ADM significantly increased cAMP levels in IM. However, inhibition of cAMP by SQ22536 further decreased ADM-attenuated osmotic water permeability. Stimulation of cAMP by roflumilast increased ADM-attenuated osmotic water permeability. Previous studies show that ADM also stimulates phospholipase C (PLC) pathways including protein kinase C (PKC) and cGMP. We tested whether PLC pathways regulate ADM-attenuated osmotic water permeability. Blockade of either PLC by U73122 or PKC by rottlerin significantly augmented the ADM-attenuated osmotic water permeability and promoted pS256-AQP2 but did change pS261-AQP2. Inhibition of cGMP by L-NAME did not change AQP2 phosphorylation. In conclusion, ADM primarily binds to the CRLR-RAMP3 receptor to initiate signaling pathways in the IM. ADM reduced water reabsorption through a PLC-pathway involving PKC. ADM-attenuated water reabsorption may be related to decreased trafficking of AQP2 to the plasma membrane. cAMP is not involved in ADM-attenuated osmotic water permeability.

UI MeSH Term Description Entries
D007679 Kidney Medulla The internal portion of the kidney, consisting of striated conical masses, the renal pyramids, whose bases are adjacent to the cortex and whose apices form prominent papillae projecting into the lumen of the minor calyces. Kidney Papilla,Kidney Medullas,Kidney Papillas,Medulla, Kidney,Medullas, Kidney,Papilla, Kidney,Papillas, Kidney
D009995 Osmosis Tendency of fluids (e.g., water) to move from the less concentrated to the more concentrated side of a semipermeable membrane. Osmoses
D010539 Permeability Property of membranes and other structures to permit passage of light, heat, gases, liquids, metabolites, and mineral ions. Permeabilities
D010766 Phosphorylation The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety. Phosphorylations
D011493 Protein Kinase C An serine-threonine protein kinase that requires the presence of physiological concentrations of CALCIUM and membrane PHOSPHOLIPIDS. The additional presence of DIACYLGLYCEROLS markedly increases its sensitivity to both calcium and phospholipids. The sensitivity of the enzyme can also be increased by PHORBOL ESTERS and it is believed that protein kinase C is the receptor protein of tumor-promoting phorbol esters. Calcium Phospholipid-Dependent Protein Kinase,Calcium-Activated Phospholipid-Dependent Kinase,PKC Serine-Threonine Kinase,Phospholipid-Sensitive Calcium-Dependent Protein Kinase,Protein Kinase M,Calcium Activated Phospholipid Dependent Kinase,Calcium Phospholipid Dependent Protein Kinase,PKC Serine Threonine Kinase,Phospholipid Sensitive Calcium Dependent Protein Kinase,Phospholipid-Dependent Kinase, Calcium-Activated,Serine-Threonine Kinase, PKC
D002462 Cell Membrane The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells. Plasma Membrane,Cytoplasmic Membrane,Cell Membranes,Cytoplasmic Membranes,Membrane, Cell,Membrane, Cytoplasmic,Membrane, Plasma,Membranes, Cell,Membranes, Cytoplasmic,Membranes, Plasma,Plasma Membranes
D003521 Cyclopropanes Three-carbon cycloparaffin cyclopropane (the structural formula (CH2)3) and its derivatives.
D000242 Cyclic AMP An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH. Adenosine Cyclic 3',5'-Monophosphate,Adenosine Cyclic 3,5 Monophosphate,Adenosine Cyclic Monophosphate,Adenosine Cyclic-3',5'-Monophosphate,Cyclic AMP, (R)-Isomer,Cyclic AMP, Disodium Salt,Cyclic AMP, Monoammonium Salt,Cyclic AMP, Monopotassium Salt,Cyclic AMP, Monosodium Salt,Cyclic AMP, Sodium Salt,3',5'-Monophosphate, Adenosine Cyclic,AMP, Cyclic,Adenosine Cyclic 3',5' Monophosphate,Cyclic 3',5'-Monophosphate, Adenosine,Cyclic Monophosphate, Adenosine,Cyclic-3',5'-Monophosphate, Adenosine,Monophosphate, Adenosine Cyclic
D000631 Aminopyridines Pyridines substituted in any position with an amino group. May be hydrogenated but must retain at least one double bond. Aminopyridine
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

Related Publications

Fuying Ma, and Guangping Chen, and Eva L Rodriguez, and Janet D Klein, and Jeff M Sands, and Yanhua Wang
Endothelin-1 inhibits AVP-stimulated osmotic water permeability in rat inner medullary collecting duct.
December 1991, The American journal of physiology,
Fuying Ma, and Guangping Chen, and Eva L Rodriguez, and Janet D Klein, and Jeff M Sands, and Yanhua Wang
Atrial natriuretic factor inhibits vasopressin-stimulated osmotic water permeability in rat inner medullary collecting duct.
October 1988, The Journal of clinical investigation,
Fuying Ma, and Guangping Chen, and Eva L Rodriguez, and Janet D Klein, and Jeff M Sands, and Yanhua Wang
Phosphatases Decrease Water and Urea Permeability in Rat Inner Medullary Collecting Ducts.
March 2023, International journal of molecular sciences,
Fuying Ma, and Guangping Chen, and Eva L Rodriguez, and Janet D Klein, and Jeff M Sands, and Yanhua Wang
Endothelin inhibits vasopressin-stimulated water permeability in rat terminal inner medullary collecting duct.
October 1992, The Journal of clinical investigation,
Fuying Ma, and Guangping Chen, and Eva L Rodriguez, and Janet D Klein, and Jeff M Sands, and Yanhua Wang
Nitric oxide inhibits ADH-stimulated osmotic water permeability in cortical collecting ducts.
January 1996, The American journal of physiology,
Fuying Ma, and Guangping Chen, and Eva L Rodriguez, and Janet D Klein, and Jeff M Sands, and Yanhua Wang
Regulation of osmotic water permeability during differentiation of inner medullary collecting duct.
May 1991, The American journal of physiology,
Fuying Ma, and Guangping Chen, and Eva L Rodriguez, and Janet D Klein, and Jeff M Sands, and Yanhua Wang
Aldosterone Decreases Vasopressin-Stimulated Water Reabsorption in Rat Inner Medullary Collecting Ducts.
April 2020, Cells,
Fuying Ma, and Guangping Chen, and Eva L Rodriguez, and Janet D Klein, and Jeff M Sands, and Yanhua Wang
PGE2 inhibits water permeability at a post-cAMP site in rat terminal inner medullary collecting duct.
February 1992, The American journal of physiology,
Fuying Ma, and Guangping Chen, and Eva L Rodriguez, and Janet D Klein, and Jeff M Sands, and Yanhua Wang
Extracellular nucleotide receptor inhibits AVP-stimulated water permeability in inner medullary collecting duct.
December 1995, The American journal of physiology,
Fuying Ma, and Guangping Chen, and Eva L Rodriguez, and Janet D Klein, and Jeff M Sands, and Yanhua Wang
Dexmedetomidine inhibits osmotic water permeability in the rat cortical collecting duct.
April 1997, The Journal of pharmacology and experimental therapeutics,
Copied contents to your clipboard!