Induction of metallothionein isoforms in cultured bovine aortic endothelial cells exposed to cadmium. 2020

Tomoya Fujie, and Yusuke Ozaki, and Fukuta Takenaka, and Misaki Nishio, and Takato Hara, and Yasuyuki Fujiwara, and Chika Yamamoto, and Toshiyuki Kaji
Department of Environmental Health, Faculty of Pharmaceutical Sciences, Toho University.

Metallothionein (MT) is an inducible protein with cytoprotective activity against heavy metals such as cadmium, zinc, and copper. MT-1 and MT-2 are the isoforms of MT induced by and bind the heavy metals. Bovine aortic endothelial cells contain three types of MT genes, namely, MT-1A, MT-1E, and MT-2A; however, the associated protein expression of these MT isoforms has not been identified. In the present study, the expression of MT subisoform proteins in cells treated with cadmium chloride was identified using a high-performance liquid chromatography-inductively coupled plasma-mass spectrometry system. It was revealed that: (1) transcriptional induction of MT-1A by cadmium was markedly more sensitive than that of MT-1E/2A; (2) MT-1A and MT-2A proteins were the predominant MT subisoforms induced by cadmium; and (3) there might be differentiation in the functions of MT-1 and MT-2 against cadmium cytotoxicity, although the actual roles of the MT isoforms in the cells were not distinct. This is the first study to show the differential induction of isoforms of MT proteins in vascular endothelial cells by cadmium.

UI MeSH Term Description Entries
D008668 Metallothionein A low-molecular-weight (approx. 10 kD) protein occurring in the cytoplasm of kidney cortex and liver. It is rich in cysteinyl residues and contains no aromatic amino acids. Metallothionein shows high affinity for bivalent heavy metals. Isometallothionein,Metallothionein A,Metallothionein B,Metallothionein I,Metallothionein II,Metallothionein IIA
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D004730 Endothelium, Vascular Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components. Capillary Endothelium,Vascular Endothelium,Capillary Endotheliums,Endothelium, Capillary,Endotheliums, Capillary,Endotheliums, Vascular,Vascular Endotheliums
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001011 Aorta The main trunk of the systemic arteries. Aortas
D015870 Gene Expression The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION. Expression, Gene,Expressions, Gene,Gene Expressions
D042783 Endothelial Cells Highly specialized EPITHELIAL CELLS that line the HEART; BLOOD VESSELS; and lymph vessels, forming the ENDOTHELIUM. They are polygonal in shape and joined together by TIGHT JUNCTIONS. The tight junctions allow for variable permeability to specific macromolecules that are transported across the endothelial layer. Capillary Endothelial Cells,Lymphatic Endothelial Cells,Vascular Endothelial Cells,Capillary Endothelial Cell,Cell, Capillary Endothelial,Cell, Endothelial,Cell, Lymphatic Endothelial,Cell, Vascular Endothelial,Cells, Capillary Endothelial,Cells, Endothelial,Cells, Lymphatic Endothelial,Cells, Vascular Endothelial,Endothelial Cell,Endothelial Cell, Capillary,Endothelial Cell, Lymphatic,Endothelial Cell, Vascular,Endothelial Cells, Capillary,Endothelial Cells, Lymphatic,Endothelial Cells, Vascular,Lymphatic Endothelial Cell,Vascular Endothelial Cell
D019256 Cadmium Chloride A cadmium halide in the form of colorless crystals, soluble in water, methanol, and ethanol. It is used in photography, in dyeing, and calico printing, and as a solution to precipitate sulfides. (McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed) CdCl2,Cadmium Dichloride,Chloride, Cadmium,Dichloride, Cadmium
D020033 Protein Isoforms Different forms of a protein that may be produced from different GENES, or from the same gene by ALTERNATIVE SPLICING. Isoform,Isoforms,Protein Isoform,Protein Splice Variant,Splice Variants, Protein,Protein Splice Variants,Isoform, Protein,Isoforms, Protein,Splice Variant, Protein,Variant, Protein Splice,Variants, Protein Splice

Related Publications

Tomoya Fujie, and Yusuke Ozaki, and Fukuta Takenaka, and Misaki Nishio, and Takato Hara, and Yasuyuki Fujiwara, and Chika Yamamoto, and Toshiyuki Kaji
January 1995, Toxicology letters,
Tomoya Fujie, and Yusuke Ozaki, and Fukuta Takenaka, and Misaki Nishio, and Takato Hara, and Yasuyuki Fujiwara, and Chika Yamamoto, and Toshiyuki Kaji
August 2016, International journal of molecular sciences,
Tomoya Fujie, and Yusuke Ozaki, and Fukuta Takenaka, and Misaki Nishio, and Takato Hara, and Yasuyuki Fujiwara, and Chika Yamamoto, and Toshiyuki Kaji
April 2016, The Journal of toxicological sciences,
Tomoya Fujie, and Yusuke Ozaki, and Fukuta Takenaka, and Misaki Nishio, and Takato Hara, and Yasuyuki Fujiwara, and Chika Yamamoto, and Toshiyuki Kaji
September 1984, Inflammation,
Tomoya Fujie, and Yusuke Ozaki, and Fukuta Takenaka, and Misaki Nishio, and Takato Hara, and Yasuyuki Fujiwara, and Chika Yamamoto, and Toshiyuki Kaji
January 1987, Arteriosclerosis (Dallas, Tex.),
Tomoya Fujie, and Yusuke Ozaki, and Fukuta Takenaka, and Misaki Nishio, and Takato Hara, and Yasuyuki Fujiwara, and Chika Yamamoto, and Toshiyuki Kaji
January 1993, Life sciences,
Tomoya Fujie, and Yusuke Ozaki, and Fukuta Takenaka, and Misaki Nishio, and Takato Hara, and Yasuyuki Fujiwara, and Chika Yamamoto, and Toshiyuki Kaji
January 2019, The Journal of toxicological sciences,
Tomoya Fujie, and Yusuke Ozaki, and Fukuta Takenaka, and Misaki Nishio, and Takato Hara, and Yasuyuki Fujiwara, and Chika Yamamoto, and Toshiyuki Kaji
April 1995, Bulletin of environmental contamination and toxicology,
Tomoya Fujie, and Yusuke Ozaki, and Fukuta Takenaka, and Misaki Nishio, and Takato Hara, and Yasuyuki Fujiwara, and Chika Yamamoto, and Toshiyuki Kaji
March 1987, Atherosclerosis,
Tomoya Fujie, and Yusuke Ozaki, and Fukuta Takenaka, and Misaki Nishio, and Takato Hara, and Yasuyuki Fujiwara, and Chika Yamamoto, and Toshiyuki Kaji
October 1994, Research communications in molecular pathology and pharmacology,
Copied contents to your clipboard!