Biomaterial Database

Rapid identification of Escherichia coli with a fluorogenic beta-glucuronidase assay. 1987

C J Papasian, and G Hertlein

Laboratory Service, Veterans Administration Medical Center, Kansas City, MO 64128.

We evaluated three fluorogenic methods (MUG; Remel, Lenexa, KS) for the rapid (less than 1 hr) identification of E. coli by detecting beta-glucuronidase activity. The methods included: direct disk inoculation test, tube test, and liquid spot reagent test. Fluorogenic tests were performed on pure cultures of lactose fermenters and compared with identification by Enterotube II (Roche Diagnostics; Nutley, NJ). Organisms yielding disparate results were further analyzed by API 20E (Analytab Products Inc.; Plainview, NY). The tube test was evaluated with isolates subcultured on both MacConkey and blood agars; the direct disk and liquid spot reagent methods were tested with isolates subcultured on blood agar only. All methods were highly specific (greater than 97%). Sensitivity of the beta-glucuronidase tests were method and media dependent, but exceeded 85% in all cases. The direct disk and tube tests also permitted detection of indole formation; results of indole testing, however, contributed little to accurately identifying E. coli.

UI	MeSH Term	Description	Entries
D004926	Escherichia coli	A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.	Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D005453	Fluorescence	The property of emitting radiation while being irradiated. The radiation emitted is usually of longer wavelength than that incident or absorbed, e.g., a substance can be irradiated with invisible radiation and emit visible light. X-ray fluorescence is used in diagnosis.
D005966	Glucuronidase		Endo-beta-D-Glucuronidase,Endoglucuronidase,Exo-beta-D-Glucuronidase,beta-Glucuronidase,Endo beta D Glucuronidase,Exo beta D Glucuronidase,beta Glucuronidase
D006923	Hymecromone	A coumarin derivative possessing properties as a spasmolytic, choleretic and light-protective agent. It is also used in ANALYTICAL CHEMISTRY TECHNIQUES for the determination of NITRIC ACID.	Imecromone,Methylumbelliferone,Resocyanine,4-Methylumbelliferone,7-Hydroxy-4-methyl-coumarin,Cholestil,Mendiaxon,4 Methylumbelliferone,7 Hydroxy 4 methyl coumarin
D001431	Bacteriological Techniques	Techniques used in studying bacteria.	Bacteriologic Technic,Bacteriologic Technics,Bacteriologic Techniques,Bacteriological Technique,Technic, Bacteriological,Technics, Bacteriological,Technique, Bacteriological,Techniques, Bacteriological,Bacteriologic Technique,Bacteriological Technic,Bacteriological Technics,Technic, Bacteriologic,Technics, Bacteriologic,Technique, Bacteriologic,Techniques, Bacteriologic

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