An in vivo intestinal perfusion technique was used to study the absorption of zinc from the duodenum, proximal jejunum and distal ileum of six dogs (group 1). Net absorption of zinc from the duodenum before and after ligation of the common bile duct averaged 596 and 574 ng.min-1.cm-1, respectively. Zinc absorption was greater (P less than 0.01) from the duodenum than from the jejunum (251 ng.min-1.cm-1) or ileum (404 ng.min-1.cm-1). Four other dogs (group 2) experienced perfusion of approximately equal segments of the duodenum (in two animals the common bile duct was ligated, and in another two it was not), proximal jejunum and distal ileum for 4 h. No change in absorption of zinc with time was noted, nor was any difference in absorption by the duodenum with and without ligation of the common bile duct observed. The data indicate that the duodenum has the greatest capacity for zinc absorption, followed by the distal ileum and proximal jejunum, and that pancreatic secretions do not appear to be necessary for adequate zinc absorption in the dog duodenum.