The Paracheirodon axelrodi is an important freshwater fish in the Amazon basin of South America, more expensive than Paracheirodon innesi. Here, we describe the complete 17,100 base pair (bp) mitochondrial genome of Paracheirodon axelrodi mohavensis. The mitogenome has a nucleotide base composition of A (29.62%), T (29.41%), G (15.39%), and C (25.62%), and encodes 13 protein subunits, 22 tRNAs, a 12S rRNA of 952 bp and 16S rRNA of 1665 bp, and a 1433 bp D-loop control region, each located in the conserved mtDNA structure typical for Characidae fishes. All protein-coding genes have initiation codons of ATG, ND1, ND2, CO2, ND3, ND4L, ND5, ND6 and Cytb ended by TAA as a stop codon, ND4 ended by AGA as a stop codon, CO1 ended by AGG as a stop codon, ATP8 and CO3 ended by TAG as a stop codon, ATP6 ended by ATT as a stop codon. This characterised mitogenome may help inform management practices for Paracheirodon axelrodi mohavensis by facilitating future studies on how allopatric populations of this imperilled species are evolving across refuge habitats.