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The complexes of T2 DNA with RNA polymerase of Escherichia coli were studied by two methods: kinetic formaldehyde method with preliminary fixation of complexes with low formaldehyde concentrations, and electron microscopy. For electron-microscopic investigations the effect of different conditions of formaldehyde fixation for DNA-RNA-polymerase complexes was studied and optimal fixation conditions were found. The suggested fixation method for DNA-RNA-polymerase complexes allows investigation of RNA polymerase molecule distribution on DNA in a wide range of conditions (ionic strength of the solution, weight ration of enzyme to DNA etc.). The comparison of the concentration of RNA polymerase molecules bound to DNA, determined by electron microscopy, and the concentration of defects in DNA as determined by the kinetic formaldehyde method, showed their coincidence. The electron-microscopic procedure was used to make maps of RNA polymerase distribution on T7 DNA. A correlation between the binding regions of the enzyme and the genetic map of early DNA T7 region was found.
D I Cherny, and
A A Aleksandrov, and
M I Zarudnaya, and
Y N Kosaganov, and
Y S Lazurkin, and
R S Beabealashvilli, and
L P Svochkina
September 1974,
FEBS letters,
D I Cherny, and
A A Aleksandrov, and
M I Zarudnaya, and
Y N Kosaganov, and
Y S Lazurkin, and
R S Beabealashvilli, and
L P Svochkina
August 1977,
The Journal of biological chemistry,
D I Cherny, and
A A Aleksandrov, and
M I Zarudnaya, and
Y N Kosaganov, and
Y S Lazurkin, and
R S Beabealashvilli, and
L P Svochkina
January 1982,
Molekuliarnaia biologiia,
D I Cherny, and
A A Aleksandrov, and
M I Zarudnaya, and
Y N Kosaganov, and
Y S Lazurkin, and
R S Beabealashvilli, and
L P Svochkina
January 1972,
Molecular biology,
D I Cherny, and
A A Aleksandrov, and
M I Zarudnaya, and
Y N Kosaganov, and
Y S Lazurkin, and
R S Beabealashvilli, and
L P Svochkina
September 1972,
Journal of molecular biology,
D I Cherny, and
A A Aleksandrov, and
M I Zarudnaya, and
Y N Kosaganov, and
Y S Lazurkin, and
R S Beabealashvilli, and
L P Svochkina
April 1976,
European journal of biochemistry,
D I Cherny, and
A A Aleksandrov, and
M I Zarudnaya, and
Y N Kosaganov, and
Y S Lazurkin, and
R S Beabealashvilli, and
L P Svochkina
December 1978,
Biochemistry,
D I Cherny, and
A A Aleksandrov, and
M I Zarudnaya, and
Y N Kosaganov, and
Y S Lazurkin, and
R S Beabealashvilli, and
L P Svochkina
September 1973,
European journal of biochemistry,
D I Cherny, and
A A Aleksandrov, and
M I Zarudnaya, and
Y N Kosaganov, and
Y S Lazurkin, and
R S Beabealashvilli, and
L P Svochkina
September 1972,
Journal of molecular biology,
D I Cherny, and
A A Aleksandrov, and
M I Zarudnaya, and
Y N Kosaganov, and
Y S Lazurkin, and
R S Beabealashvilli, and
L P Svochkina
November 1981,
European journal of biochemistry,
