-transparent.png){kind=logo}
The extent of methylation of the c-H-ras and c-K-ras oncogenes was compared in neoplastic and preneoplastic livers of rats fed one of several methyl-deficient, amino acid-defined diets for 18 months, with or without a preceding initiating dose of diethylnitrosamine (DEN). The restriction endonucleases MspI, HpaII, HhaI, PaeR71 and XhoI were used for studying the extent and pattern of DNA methylation. The results indicated that both c-H-ras and c-K-ras oncogenes were hypomethylated in all DNA samples derived from both neoplastic and preneoplastic livers of rats fed any of the methyl-deficient diets used, regardless of whether or not the rats had received an initiating dose of DEN. It thus appears that dietary methyl deficiency does indeed lead to hypomethylation of ras genes in the DNAs of the resulting tumors. However, the significance of this hypomethylation in the tumorigenic process is not clearly understood.
M R Bhave, and
M J Wilson, and
L A Poirier
October 1992,
Carcinogenesis,
M R Bhave, and
M J Wilson, and
L A Poirier
December 1983,
Carcinogenesis,
M R Bhave, and
M J Wilson, and
L A Poirier
January 1983,
The Journal of nutrition,
M R Bhave, and
M J Wilson, and
L A Poirier
August 1983,
Carcinogenesis,
M R Bhave, and
M J Wilson, and
L A Poirier
August 1989,
Cancer research,
M R Bhave, and
M J Wilson, and
L A Poirier
April 1993,
Cancer letters,
M R Bhave, and
M J Wilson, and
L A Poirier
July 1964,
Archives of biochemistry and biophysics,
M R Bhave, and
M J Wilson, and
L A Poirier
September 1989,
Nucleic acids research,
M R Bhave, and
M J Wilson, and
L A Poirier
February 1975,
The Journal of nutrition,
M R Bhave, and
M J Wilson, and
L A Poirier
January 1947,
Federation proceedings,
