Production of monoclonal antibody against Aeromonas hydrophila haemolysin. 1988

S Kozaki, and K Kato, and A Kurokawa, and Y Kamata, and T Asao, and G Sakaguchi
Department of Veterinary Science, College of Agriculture, University of Osaka Prefecture, Japan.

Two hybridoma cell lines that produce monoclonal antibodies against Aeromonas hydrophila haemolysin were established by fusion of myeloma and spleen cells obtained from a mouse immunised with haemolysin detoxified with tetranitromethane. Enzyme-linked immunosorbent assay (ELISA) showed that the two purified monoclonal antibodies, B7 and B11, recognised the same epitope on the haemolysin molecule. Antibody B7 neutralised the haemolytic and enterotoxic activities of the haemolysin. It is concluded that the same site on the haemolysin molecule is responsible for both haemolytic and enterotoxic activities.

UI MeSH Term Description Entries
D008807 Mice, Inbred BALB C An inbred strain of mouse that is widely used in IMMUNOLOGY studies and cancer research. BALB C Mice, Inbred,BALB C Mouse, Inbred,Inbred BALB C Mice,Inbred BALB C Mouse,Mice, BALB C,Mouse, BALB C,Mouse, Inbred BALB C,BALB C Mice,BALB C Mouse
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D006460 Hemolysin Proteins Proteins from BACTERIA and FUNGI that are soluble enough to be secreted to target ERYTHROCYTES and insert into the membrane to form beta-barrel pores. Biosynthesis may be regulated by HEMOLYSIN FACTORS. Hemolysin,Hemolysins,Hemalysins,Proteins, Hemolysin
D006825 Hybridomas Cells artificially created by fusion of activated lymphocytes with neoplastic cells. The resulting hybrid cells are cloned and produce pure MONOCLONAL ANTIBODIES or T-cell products, identical to those produced by the immunologically competent parent cell. Hybridoma
D000333 Aeromonas A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria that occurs singly, in pairs, or in short chains. Its organisms are found in fresh water and sewage and are pathogenic to humans, frogs, and fish.
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000911 Antibodies, Monoclonal Antibodies produced by a single clone of cells. Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D051379 Mice The common name for the genus Mus. Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus

Related Publications

S Kozaki, and K Kato, and A Kurokawa, and Y Kamata, and T Asao, and G Sakaguchi
January 2002, Journal of applied microbiology,
S Kozaki, and K Kato, and A Kurokawa, and Y Kamata, and T Asao, and G Sakaguchi
December 1992, Microbial pathogenesis,
S Kozaki, and K Kato, and A Kurokawa, and Y Kamata, and T Asao, and G Sakaguchi
August 1989, Journal of clinical microbiology,
S Kozaki, and K Kato, and A Kurokawa, and Y Kamata, and T Asao, and G Sakaguchi
January 1991, Research in microbiology,
S Kozaki, and K Kato, and A Kurokawa, and Y Kamata, and T Asao, and G Sakaguchi
April 1976, Acta pathologica et microbiologica Scandinavica. Section B, Microbiology,
S Kozaki, and K Kato, and A Kurokawa, and Y Kamata, and T Asao, and G Sakaguchi
January 2022, Frontiers in veterinary science,
S Kozaki, and K Kato, and A Kurokawa, and Y Kamata, and T Asao, and G Sakaguchi
October 2002, Die Nahrung,
S Kozaki, and K Kato, and A Kurokawa, and Y Kamata, and T Asao, and G Sakaguchi
September 1973, Journal of general microbiology,
S Kozaki, and K Kato, and A Kurokawa, and Y Kamata, and T Asao, and G Sakaguchi
June 1984, Japanese journal of medical science & biology,
S Kozaki, and K Kato, and A Kurokawa, and Y Kamata, and T Asao, and G Sakaguchi
August 1986, European journal of clinical microbiology,
Copied contents to your clipboard!