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The primary structure of a mutant lipoprotein of the outer membrane of Escherichia coli was investigated. This mutant was previously described as a mutant that forms a dimer of the lipoprotein by an S-S bridge (H. Suzuki et al., J. Bacteriol. 127:1494-1501, 1976). The amino acid analysis of the mutant lipoprotein revealed that the mutant lipoprotein had an extra cysteine residue, with concomitant loss of an arginine residue. From the analysis of the mutant lipoprotein revealed that the mutant lipoprotein had an extra cysteine residue, with concomitant loss of an arginine residue. From the analysis of tryptic peptides, it was found that the arginine residue at position 57 was replaced with a cysteine residue. The amino terminal structure of the mutant lipoprotein was found to be glycerylcysteine, as in the case of the wild-type lipoprotein. The present results show that the mutation that was previously determined to map at 36.5 min on the E. coli chromosome occurred in the structure gene (lpp) for the lipoprotein. This was further confirmed by the fact that a merodiploid carrying both lpp+ and lpp produces not only the wild-type lipoprotein but also the mutant lipoprotein.
S Inouye, and
N Lee, and
M Inouye, and
H C Wu, and
H Suzuki, and
Y Nishimura, and
H Iketani, and
Y Hirota
February 1980,
Journal of bacteriology,
S Inouye, and
N Lee, and
M Inouye, and
H C Wu, and
H Suzuki, and
Y Nishimura, and
H Iketani, and
Y Hirota
December 1982,
Journal of bacteriology,
S Inouye, and
N Lee, and
M Inouye, and
H C Wu, and
H Suzuki, and
Y Nishimura, and
H Iketani, and
Y Hirota
January 1979,
Biomembranes,
S Inouye, and
N Lee, and
M Inouye, and
H C Wu, and
H Suzuki, and
Y Nishimura, and
H Iketani, and
Y Hirota
January 1980,
The Journal of biological chemistry,
S Inouye, and
N Lee, and
M Inouye, and
H C Wu, and
H Suzuki, and
Y Nishimura, and
H Iketani, and
Y Hirota
February 1975,
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
S Inouye, and
N Lee, and
M Inouye, and
H C Wu, and
H Suzuki, and
Y Nishimura, and
H Iketani, and
Y Hirota
October 1978,
Proceedings of the National Academy of Sciences of the United States of America,
S Inouye, and
N Lee, and
M Inouye, and
H C Wu, and
H Suzuki, and
Y Nishimura, and
H Iketani, and
Y Hirota
October 1975,
Biochimica et biophysica acta,
S Inouye, and
N Lee, and
M Inouye, and
H C Wu, and
H Suzuki, and
Y Nishimura, and
H Iketani, and
Y Hirota
February 1986,
The Journal of biological chemistry,
S Inouye, and
N Lee, and
M Inouye, and
H C Wu, and
H Suzuki, and
Y Nishimura, and
H Iketani, and
Y Hirota
February 1987,
Journal of molecular biology,
S Inouye, and
N Lee, and
M Inouye, and
H C Wu, and
H Suzuki, and
Y Nishimura, and
H Iketani, and
Y Hirota
February 1984,
Proceedings of the National Academy of Sciences of the United States of America,
