Prevalence of Borrelia burgdorferi and Anaplasma phagocytophilum in Ixodid Ticks from Southeastern Ukraine. 2021

Nadia Kovryha, and Ala Tsyhankova, and Olena Zelenuchina, and Olexandr Mashchak, and Roman Terekhov, and Artem S Rogovskyy
The Zaporizhzhya Oblast Laboratory Center, the Ministry of Health of Ukraine, Zaporizhzhya, Ukraine.

Tick-borne diseases have emerged as an increasing medical problem in the world. Being the most prevalent ixodid ticks in Europe, Ixodes ricinus and Dermacentor reticulatus are responsible for transmission of numerous zoonotic pathogens (e.g., human granulocytic anaplasmosis and Lyme borreliosis). Despite their public health significance, studies on the prevalence of tick-borne agents are scare for Eastern Europe. The objective of this study was to examine the prevalence of Anaplasma phagocytophilum, Ehrlichia chaffeensis, and Borrelia burgdorferi sensu lato (B. burgdorferi s. l.) in ixodid ticks from Southeastern Ukraine. Over a 5-year period (2014-2018), 358 questing and 389 engorged ixodid ticks were collected from Southeastern Ukraine (Zaporizhzhya region). The ticks were identified as Dermacentor marginatus, D. reticulatus, I. ricinus, and Rhipicephalus rossicus. Nucleic acid samples extracted from tick pools were subjected to RT-PCR analyses for A. phagocytophilum, E. chaffeensis, and B. burgdorferi s. l. The examined ixodid ticks tested negative for the aforementioned pathogens with the exception of I. ricinus ticks. For questing I. ricinus ticks, minimum infection rates of A. phagocytophilum and B. burgdorferi s. l. were, respectively, 4.2-7.7% and 8.6-12.7%. These findings will be valuable for medical and veterinary practitioners when risks associated with tick-borne diseases are assessed for southeastern regions of Ukraine.

UI MeSH Term Description Entries
D001898 Borrelia A genus of gram-negative, anaerobic, helical bacteria, various species of which produce RELAPSING FEVER in humans and other animals.
D003870 Dermacentor A widely distributed genus of TICKS, in the family IXODIDAE, including a number that infest humans and other mammals. Several are vectors of diseases such as TULAREMIA; ROCKY MOUNTAIN SPOTTED FEVER; COLORADO TICK FEVER; and ANAPLASMOSIS. Dermacentor andersoni,Rocky Mountain Wood Tick
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012281 Rickettsia A genus of gram-negative, aerobic, rod-shaped bacteria often surrounded by a protein microcapsular layer and slime layer. The natural cycle of its organisms generally involves a vertebrate and an invertebrate host. Species of the genus are the etiological agents of human diseases, such as typhus.
D014455 Ukraine Country in Europe, bordering the Black Sea, between Poland, Romania, and Moldova in the west and Russia in the east. The capital is Kyiv.
D015748 Borrelia burgdorferi Group Gram-negative helical bacteria, in the genus BORRELIA, that are the etiologic agents of LYME DISEASE. The group comprises many specific species including Borrelia afzelii, Borellia garinii, and BORRELIA BURGDORFERI proper. These spirochetes are generally transmitted by several species of ixodid ticks. Borrelia afzelii,Borrelia burgdorferi sensu lato,Borrelia garinii,Borreliella afzelii,Borreliella garinii
D015995 Prevalence The total number of cases of a given disease in a specified population at a designated time. It is differentiated from INCIDENCE, which refers to the number of new cases in the population at a given time. Period Prevalence,Point Prevalence,Period Prevalences,Point Prevalences,Prevalence, Period,Prevalence, Point,Prevalences
D018884 Ixodes The largest genus of TICKS in the family IXODIDAE, containing over 200 species. Many infest humans and other mammals and several are vectors of diseases such as LYME DISEASE; ENCEPHALITIS, TICK-BORNE; and KYASANUR FOREST DISEASE. Black-legged Tick,Blacklegged Tick,California Black Legged Tick,Castor Bean Tick,Deer Tick,Ixodes dammini,Ixodes pacificus,Ixodes persulcatus,Ixodes ricinus,Ixodes scapularis,Western Black Legged Tick,Western Blacklegged Tick,Black legged Tick,Black-legged Ticks,Blacklegged Tick, Western,Blacklegged Ticks,Castor Bean Ticks,Deer Ticks,Ixodes damminus,Ixodes scapulari,Tick, Black-legged,Tick, Blacklegged,Tick, Castor Bean,Tick, Deer,Tick, Western Blacklegged,Western Blacklegged Ticks
D025065 Borrelia burgdorferi A specific species of bacteria, part of the BORRELIA BURGDORFERI GROUP, whose common name is Lyme disease spirochete. Borrelia burgdorferi sensu stricto,Lyme Disease Spirochete
D041081 Anaplasma phagocytophilum A species of gram-negative bacteria in the genus ANAPLASMA, family ANAPLASMATACEAE, formerly called Ehrlichia phagocytophila or Ehrlichia equi. This organism is tick-borne (IXODES) and causes disease in horses and sheep. In humans, it causes human granulocytic EHRLICHIOSIS. Cytoecetes phagocytophila,Ehrlichia equi,Ehrlichia phagocytophila,HGE Agent

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