Biomaterial Database

Cl(-)-HCO3- exchange is present with Na+-K+-Cl- cotransport in rabbit parotid acinar basolateral membranes. 1988

R J Turner, and J N George

Clinical Investigations and Patient Care Branch, National Institute of Dental Research, Bethesda, Maryland 20892.

The presence of a sodium-independent electroneutral Cl(-)-anion exchanger in a basolateral membrane vesicle preparation from the rabbit parotid is demonstrated. This exchanger is shared by HCO3-, NO3-, Br-, F-, and formate, but not by thiocyanate, acetate, methylsulfate, gluconate, or hydroxyl ions. In order of relative potency, the exchanger is inhibited by SITS greater than or equal to phloretin greater than furosemide greater than bumetanide greater than or equal to phlorizin. A Na+-K+-dependent component of chloride flux, presumably due to the Na+-K+-Cl- cotransporter already characterized in this preparation, was also observed. 36Cl uptake into vesicles loaded with KCl exhibited an "overshoot" of intravesicular [36Cl] due to 36Cl-Cl exchange. However, when vesicles were loaded with both KCl and NaCl the height of the overshoot was considerably decreased indicating a Na+-K+-dependent dissipation of the intravesicular to extravesicular chloride gradient. This experiment provides strong evidence that the Na+-K+-Cl- cotransporter and the Cl(-)-HCO3- exchanger are present in the same membrane vesicles. These results indicate that Cl(-)-HCO3- exchange is present in the basolateral membrane of parotid acinar cells and thus that this transporter may play a significant role in salivary secretion.

UI	MeSH Term	Description	Entries
D007474	Ion Exchange	Reversible chemical reaction between a solid, often one of the ION EXCHANGE RESINS, and a fluid whereby ions may be exchanged from one substance to another. This technique is used in water purification, in research, and in industry.	Exchange, Ion
D008566	Membranes	Thin layers of tissue which cover parts of the body, separate adjacent cavities, or connect adjacent structures.	Membrane Tissue,Membrane,Membrane Tissues,Tissue, Membrane,Tissues, Membrane
D010306	Parotid Gland	The largest of the three pairs of SALIVARY GLANDS. They lie on the sides of the FACE immediately below and in front of the EAR.	Gland, Parotid,Glands, Parotid,Parotid Glands
D011817	Rabbits	A burrowing plant-eating mammal with hind limbs that are longer than its fore limbs. It belongs to the family Leporidae of the order Lagomorpha, and in contrast to hares, possesses 22 instead of 24 pairs of chromosomes.	Belgian Hare,New Zealand Rabbit,New Zealand Rabbits,New Zealand White Rabbit,Rabbit,Rabbit, Domestic,Chinchilla Rabbits,NZW Rabbits,New Zealand White Rabbits,Oryctolagus cuniculus,Chinchilla Rabbit,Domestic Rabbit,Domestic Rabbits,Hare, Belgian,NZW Rabbit,Rabbit, Chinchilla,Rabbit, NZW,Rabbit, New Zealand,Rabbits, Chinchilla,Rabbits, Domestic,Rabbits, NZW,Rabbits, New Zealand,Zealand Rabbit, New,Zealand Rabbits, New,cuniculus, Oryctolagus
D002352	Carrier Proteins	Proteins that bind or transport specific substances in the blood, within the cell, or across cell membranes.	Binding Proteins,Carrier Protein,Transport Protein,Transport Proteins,Binding Protein,Protein, Carrier,Proteins, Carrier
D002712	Chlorides	Inorganic compounds derived from hydrochloric acid that contain the Cl- ion.	Chloride,Chloride Ion Level,Ion Level, Chloride,Level, Chloride Ion
D004563	Electrochemistry	The study of chemical changes resulting from electrical action and electrical activity resulting from chemical changes.	Electrochemistries
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000838	Anions	Negatively charged atoms, radicals or groups of atoms which travel to the anode or positive pole during electrolysis.	Anion
D027963	Chloride-Bicarbonate Antiporters	Electroneutral chloride bicarbonate exchangers that allow the exchange of BICARBONATE IONS exchange for CHLORIDE IONS across the cellular membrane. The action of specific antiporters in this class serve important functions such as allowing the efficient exchange of bicarbonate across red blood cell membranes as they passage through capillaries and the reabsorption of bicarbonate ions by the kidney.	AE Proteins,AE2 Anion Exchanger,AE2 Chloride-Bicarbonate Exchanger,AE3 Anion Exchanger,AE4 Anion Exchanger,Anion Exchange Protein 2,Anion Exchange Protein 3,Anion Exchange Protein 4,Anion Exchanger 2,Anion Exchanger 3,Anion Exchanger 4,Bicarbonate-Chloride Antiport,Cardiac Brain Band 3-Like Protein,Chloride-Bicarbonate Exchanger,Chloride-Bicarbonate Exchangers,Cl(-)-HCO3(-)-Exchanger,Cl- HCO3- Exchanger,Cl- HCO3- Exchangers,Neuronal Band 3-Like Protein,Non-Erythroid Band 3-Like Protein,SLC4A2 Protein,SLC4A3 Protein,SLC4A9 Protein,Sodium Bicarbonate Cotransporter 5,Solute Carrier Family 4 Member 2,Solute Carrier Family 4 Member 3,Solute Carrier Family 4 Member 9,Solute Carrier Family 4, Sodium Bicarbonate Cotransporter, Member 9,AE2 Chloride Bicarbonate Exchanger,Anion Exchanger, AE2,Anion Exchanger, AE3,Anion Exchanger, AE4,Antiport, Bicarbonate-Chloride,Antiporters, Chloride-Bicarbonate,Bicarbonate Chloride Antiport,Cardiac Brain Band 3 Like Protein,Chloride Bicarbonate Antiporters,Chloride Bicarbonate Exchanger,Chloride Bicarbonate Exchangers,Chloride-Bicarbonate Exchanger, AE2,Cl HCO3 Exchanger,Cl HCO3 Exchangers,Exchanger, Chloride-Bicarbonate,Exchangers, Chloride-Bicarbonate,Exchangers, Cl- HCO3-,HCO3- Exchangers, Cl-,Neuronal Band 3 Like Protein,Non Erythroid Band 3 Like Protein,Protein, SLC4A2,Protein, SLC4A3,Protein, SLC4A9,Proteins, AE