-transparent.png){kind=logo}
Protein tyrosine phosphorylation in rabbit peritoneal neutrophils was examined by immunoblotting with antibodies specific for phosphotyrosine. Two tyrosine phosphorylated proteins were found with apparent molecular weights of 62,000 (p62) and 125,000. Both were enriched in the membrane fraction. Stimulation of the neutrophils with chemotactic factor fMet-Leu-Phe (10(-8)M, 20 sec) but not phorbol 12-myristate-13-acetate (0.1 microgram/ml, 10 min) caused rapid increase in tyrosine phosphorylation. The effect of fMet-Leu-Phe was inhibited by the pretreatment of neutrophils with pertussis toxin. The p62 protein was also recognized by antibody raised against a synthetic fragment commonly found in the tyrosine kinases of the src gene family. The results indicate that stimulation of the tyrosine phosphorylation of membrane associated proteins is one of the early events occurring in activated neutrophil and this stimulation of tyrosine phosphorylation may be regulated by a GTP-binding protein.
C K Huang, and
G R Laramee, and
J E Casnellie
February 1979,
Science (New York, N.Y.),
C K Huang, and
G R Laramee, and
J E Casnellie
February 1984,
The Journal of biological chemistry,
C K Huang, and
G R Laramee, and
J E Casnellie
September 1980,
Inflammation,
C K Huang, and
G R Laramee, and
J E Casnellie
November 1980,
Biochimica et biophysica acta,
C K Huang, and
G R Laramee, and
J E Casnellie
May 1991,
The American journal of physiology,
C K Huang, and
G R Laramee, and
J E Casnellie
October 1986,
Archives of biochemistry and biophysics,
C K Huang, and
G R Laramee, and
J E Casnellie
November 1993,
Biochemical Society transactions,
C K Huang, and
G R Laramee, and
J E Casnellie
January 1981,
Kroc Foundation series,
C K Huang, and
G R Laramee, and
J E Casnellie
August 1992,
Proceedings of the National Academy of Sciences of the United States of America,
C K Huang, and
G R Laramee, and
J E Casnellie
August 1997,
British journal of pharmacology,
