Commercial Silver Diamine Fluoride (SDF) Products on Caries Lesion Progression in Primary Enamel: An In Vitro Study. 2020

Dayse A Romão, and Constanza E Fernández, and Lucineide de Melo Santos

Purpose: Evidence has shown that silver diamine fluoride (SDF) at 30-38% has the potential to control and revert caries lesions. However, SDF can be found at lower concentrations on the market. In this study, we evaluated the effect of different commercially available SDF products on the progression of non-cavitated caries lesion in primary teeth assessed by a pH-cycling model. Materials and Methods: Subsuperficial caries lesions were formed in primary teeth using a demineralising solution for 96 h. Demineralised samples were randomly allocated to the following groups (n = 12/group): (G1) Negative Control, deionised water; (G2) Cariostatic, Inodon; 10%* SDF; (G3) Cariestop, Biodynamics, 12%* SDF; (G4) Cariostal, Iodontec, 16%* SDF; (G5) Cariestop, Biodynamics, 30%* SDF (*concentrations declared in the label). Products were applied according to the manufacturer's recommendation and reapplied after 7 days. Samples were exposed to a pH-cycling model for 14 days. After the cycle was completed, samples were sectioned, analysed by polarised light microscopy, and lesion depth was estimated as indicator of caries lesion progression. Groups were compared by multiple comparisons test (p < 0.05). Results: The negative control group exhibited the greatest lesion depth. All SDF products reduced the caries lesions depth and differed from the negative control. It was a trend observed (G1>G2>G3>G4), but no statistical differences among G3, and G5, and between G4 and G5 were observed for lesion depth. Conclusion: The tested SDF products reduced the depth of non-cavitated carious lesions in primary enamel. Although SDF products with declared concentrations between 12% and 30% (G3, G4, and G5) demonstrated a similar lesion depth in primary enamel, the effect of the concentration remains unclear.

UI MeSH Term Description Entries
D002327 Cariostatic Agents Substances that inhibit or arrest DENTAL CARIES formation. (Boucher's Clinical Dental Terminology, 4th ed) Cariostatic Effect,Cariostatic Effects,Agent, Cariostatic,Agents, Cariostatic,Cariostatic Agent,Effect, Cariostatic,Effects, Cariostatic
D003731 Dental Caries Localized destruction of the tooth surface initiated by decalcification of the enamel followed by enzymatic lysis of organic structures and leading to cavity formation. If left unchecked, the cavity may penetrate the enamel and dentin and reach the pulp. Caries, Dental,Carious Lesions,Dental Cavities,Dental Cavity,Dental Decay,Dental White Spots,Carious Dentin,Decay, Dental,Dental White Spot,White Spot, Dental,White Spots, Dental,Carious Dentins,Carious Lesion,Cavities, Dental,Cavity, Dental,Dentin, Carious,Dentins, Carious,Lesion, Carious,Lesions, Carious,Spot, Dental White,Spots, Dental White
D003743 Dental Enamel A hard thin translucent layer of calcified substance which envelops and protects the dentin of the crown of the tooth. It is the hardest substance in the body and is almost entirely composed of calcium salts. Under the microscope, it is composed of thin rods (enamel prisms) held together by cementing substance, and surrounded by an enamel sheath. (From Jablonski, Dictionary of Dentistry, 1992, p286) Enamel,Enamel Cuticle,Dental Enamels,Enamel, Dental,Enamels, Dental,Cuticle, Enamel,Cuticles, Enamel,Enamel Cuticles,Enamels
D005460 Fluorides, Topical Fluorides, usually in pastes or gels, used for topical application to reduce the incidence of DENTAL CARIES. Fluoride Varnishes,Topical Fluorides,Varnishes, Fluoride
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000644 Quaternary Ammonium Compounds Derivatives of ammonium compounds, NH4+ Y-, in which all four of the hydrogens bonded to nitrogen have been replaced with hydrocarbyl groups. These are distinguished from IMINES which are RN Quaternary Ammonium Compound,Ammonium Compound, Quaternary,Ammonium Compounds, Quaternary,Compound, Quaternary Ammonium
D018030 Silver Compounds Inorganic compounds that contain silver as an integral part of the molecule. Compounds, Silver

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