Cyanogenic glycoside amygdalin influences functions of human osteoblasts in vitro. 2021

Radoslav Omelka, and Veronika Kovacova, and Vladimira Mondockova, and Birgit Grosskopf, and Adriana Kolesarova, and Monika Martiniakova
Department of Botany and Genetics, Faculty of Natural Sciences, Constantine the Philosopher University in Nitra, Nitra, Slovak Republic.

Amygdalin has been promoted as an alternative cancer cure. However, it is still unclear how this cyanogenic glycoside affects non-cancer cells including bone cells. This study first investigated the impact of amygdalin on viability, morphology and expression of important genes in human osteoblasts in vitro. Primary human osteoblast cultures were exposed to amygdalin at concentrations 0; 0.1; 1 and 10 mg/mL in growth medium for 72 h. Cell viability, osteoblasts morphology and expression of 10 genes associated with osteoblast-specific pathways, oxidative stress and cell death were determined. Osteoblasts viability was significantly decreased (-27.26%) and their size was reduced (-23.20%) at the highest concentration of amygdalin (10 mg/mL). This concentration of amygdalin down-regulated the expression of COL1A1 and ALPL genes, whereas the expression of BGLAP, TNFSF11 and WNT5A genes was increased. The osteoblast cultivation with 0.1 mg/mL amygdalin caused down-regulation of COL1A1 gene. No changes in expression were determined for RUNX2, BAX, CASP1, SOD1 and GPX1 genes among all tested concentrations of amygdalin. In conclusion, amygdalin in a high concentration negatively affected mineralization of extracellular matrix, increased bone resorption and decreased osteoblast viability. These changes were accompanied by modified expression profiles of responsible genes.

UI MeSH Term Description Entries
D010006 Osteoblasts Bone-forming cells which secrete an EXTRACELLULAR MATRIX. HYDROXYAPATITE crystals are then deposited into the matrix to form bone. Osteoblast
D002470 Cell Survival The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability. Cell Viability,Cell Viabilities,Survival, Cell,Viabilities, Cell,Viability, Cell
D004305 Dose-Response Relationship, Drug The relationship between the dose of an administered drug and the response of the organism to the drug. Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response
D006027 Glycosides Any compound that contains a constituent sugar, in which the hydroxyl group attached to the first carbon is substituted by an alcoholic, phenolic, or other group. They are named specifically for the sugar contained, such as glucoside (glucose), pentoside (pentose), fructoside (fructose), etc. Upon hydrolysis, a sugar and nonsugar component (aglycone) are formed. (From Dorland, 28th ed; From Miall's Dictionary of Chemistry, 5th ed) Glycoside
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000678 Amygdalin A cyanogenic glycoside found in the seeds of Rosaceae. Amygdaloside,Mandelonitrile-beta-Gentiobioside,Vitamin B17,Laetrile,Neoamygdalin,Mandelonitrile beta Gentiobioside
D000972 Antineoplastic Agents, Phytogenic Agents obtained from higher plants that have demonstrable cytostatic or antineoplastic activity. Antineoplastics, Botanical,Antineoplastics, Phytogenic,Agents, Phytogenic Antineoplastic,Botanical Antineoplastics,Phytogenic Antineoplastic Agents,Phytogenic Antineoplastics
D015870 Gene Expression The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION. Expression, Gene,Expressions, Gene,Gene Expressions
D016923 Cell Death The termination of the cell's ability to carry out vital functions such as metabolism, growth, reproduction, responsiveness, and adaptability. Death, Cell
D018384 Oxidative Stress A disturbance in the prooxidant-antioxidant balance in favor of the former, leading to potential damage. Indicators of oxidative stress include damaged DNA bases, protein oxidation products, and lipid peroxidation products (Sies, Oxidative Stress, 1991, pxv-xvi). Anti-oxidative Stress,Antioxidative Stress,DNA Oxidative Damage,Nitro-Oxidative Stress,Oxidative Cleavage,Oxidative DNA Damage,Oxidative Damage,Oxidative Injury,Oxidative Nitrative Stress,Oxidative Stress Injury,Oxidative and Nitrosative Stress,Stress, Oxidative,Anti oxidative Stress,Anti-oxidative Stresses,Antioxidative Stresses,Cleavage, Oxidative,DNA Damage, Oxidative,DNA Oxidative Damages,Damage, DNA Oxidative,Damage, Oxidative,Damage, Oxidative DNA,Injury, Oxidative,Injury, Oxidative Stress,Nitrative Stress, Oxidative,Nitro Oxidative Stress,Nitro-Oxidative Stresses,Oxidative Cleavages,Oxidative DNA Damages,Oxidative Damage, DNA,Oxidative Damages,Oxidative Injuries,Oxidative Nitrative Stresses,Oxidative Stress Injuries,Oxidative Stresses,Stress Injury, Oxidative,Stress, Anti-oxidative,Stress, Antioxidative,Stress, Nitro-Oxidative,Stress, Oxidative Nitrative,Stresses, Nitro-Oxidative

Related Publications

Radoslav Omelka, and Veronika Kovacova, and Vladimira Mondockova, and Birgit Grosskopf, and Adriana Kolesarova, and Monika Martiniakova
October 2021, Molecules (Basel, Switzerland),
Radoslav Omelka, and Veronika Kovacova, and Vladimira Mondockova, and Birgit Grosskopf, and Adriana Kolesarova, and Monika Martiniakova
November 2022, Molecules (Basel, Switzerland),
Radoslav Omelka, and Veronika Kovacova, and Vladimira Mondockova, and Birgit Grosskopf, and Adriana Kolesarova, and Monika Martiniakova
November 1995, Human & experimental toxicology,
Radoslav Omelka, and Veronika Kovacova, and Vladimira Mondockova, and Birgit Grosskopf, and Adriana Kolesarova, and Monika Martiniakova
March 2019, Journal of animal physiology and animal nutrition,
Radoslav Omelka, and Veronika Kovacova, and Vladimira Mondockova, and Birgit Grosskopf, and Adriana Kolesarova, and Monika Martiniakova
March 1967, Indian journal of biochemistry,
Radoslav Omelka, and Veronika Kovacova, and Vladimira Mondockova, and Birgit Grosskopf, and Adriana Kolesarova, and Monika Martiniakova
July 2014, Journal of agricultural and food chemistry,
Radoslav Omelka, and Veronika Kovacova, and Vladimira Mondockova, and Birgit Grosskopf, and Adriana Kolesarova, and Monika Martiniakova
June 1975, The Journal of biological chemistry,
Radoslav Omelka, and Veronika Kovacova, and Vladimira Mondockova, and Birgit Grosskopf, and Adriana Kolesarova, and Monika Martiniakova
March 2021, Molecules (Basel, Switzerland),
Radoslav Omelka, and Veronika Kovacova, and Vladimira Mondockova, and Birgit Grosskopf, and Adriana Kolesarova, and Monika Martiniakova
January 2014, PloS one,
Radoslav Omelka, and Veronika Kovacova, and Vladimira Mondockova, and Birgit Grosskopf, and Adriana Kolesarova, and Monika Martiniakova
June 1996, Phytochemistry,
Copied contents to your clipboard!