BIOMAT Database Logo BIOMAT Database Logo

Immunoreactive somatomedin C/insulin-like growth factor I in urine from normal subjects, pituitary dwarfs, and acromegalics. 1988

S Yokoya, and S Suwa, and H Maesaka, and T Tanaka
Department of Pediatrics, Kanagawa Children's Medical Center, Yokohama, Japan.

Using antibodies to somatomedin C/insulin-like growth factor I (SmC) produced in rabbits using the recombinant hormone, we have developed a radioimmunoassay for SmC. Gel-chromatography of urine revealed that the vast majority of immunoreactive SmC was eluted coincident with 125I-SmC and a small portion eluted with fractions having a mol. wt. range of 30,000-40,000. The SmC concentration in urine was determined by radioimmunoassay after ammonium sulfate extraction. Values did not ordinarily exceed 1 ng/ml. When the values from normal subjects were expressed as ng/mg creatinine, high levels were observed in the neonatal period. These values fell rapidly in infancy, declined more gradually in childhood, were slightly elevated at early puberty, and were lowest in adulthood. Urine SmC concentrations in 15 pituitary dwarfs were lower than the averages obtained from agematched control subjects, and six of them showed abnormally low values. Three patients with active acromegaly had high SmC values in urine. In conclusion, 1) SmC, mainly of monomeric form, was immunologically detected in urine. 2) Radioimmunoassay for urine SmC revealed that values varied considerably with age in normal subjects and were partially dependent on the human growth hormone status. However, the full meaning of the findings remains to be elucidated.

UI MeSH Term Description Entries
D007223 Infant A child between 1 and 23 months of age. Infants
D007231 Infant, Newborn An infant during the first 28 days after birth. Neonate,Newborns,Infants, Newborn,Neonates,Newborn,Newborn Infant,Newborn Infants
D007334 Insulin-Like Growth Factor I A well-characterized basic peptide believed to be secreted by the liver and to circulate in the blood. It has growth-regulating, insulin-like, and mitogenic activities. This growth factor has a major, but not absolute, dependence on GROWTH HORMONE. It is believed to be mainly active in adults in contrast to INSULIN-LIKE GROWTH FACTOR II, which is a major fetal growth factor. IGF-I,Somatomedin C,IGF-1,IGF-I-SmC,Insulin Like Growth Factor I,Insulin-Like Somatomedin Peptide I,Insulin Like Somatomedin Peptide I
D008297 Male Males
D011863 Radioimmunoassay Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation. Radioimmunoassays
D012016 Reference Values The range or frequency distribution of a measurement in a population (of organisms, organs or things) that has not been selected for the presence of disease or abnormality. Normal Range,Normal Values,Reference Ranges,Normal Ranges,Normal Value,Range, Normal,Range, Reference,Ranges, Normal,Ranges, Reference,Reference Range,Reference Value,Value, Normal,Value, Reference,Values, Normal,Values, Reference
D002648 Child A person 6 to 12 years of age. An individual 2 to 5 years old is CHILD, PRESCHOOL. Children
D002675 Child, Preschool A child between the ages of 2 and 5. Children, Preschool,Preschool Child,Preschool Children
D004393 Dwarfism, Pituitary A form of dwarfism caused by complete or partial GROWTH HORMONE deficiency, resulting from either the lack of GROWTH HORMONE-RELEASING FACTOR from the HYPOTHALAMUS or from the mutations in the growth hormone gene (GH1) in the PITUITARY GLAND. It is also known as Type I pituitary dwarfism. Human hypophysial dwarf is caused by a deficiency of HUMAN GROWTH HORMONE during development. Growth Hormone Deficiency Dwarfism,Hypophysial Dwarf,Hyposomatotrophic Dwarfism,Pituitary Dwarf,Dwarfism, Growth Hormone Deficiency,Isolated GH Deficiency,Isolated Growth Hormone Deficiency,Isolated HGH Deficiency,Isolated Human Growth Hormone Deficiency,Isolated Somatotropin Deficiency,Isolated Somatotropin Deficiency Disorder,Nanism, Pituitary,Pituitary Dwarfism,Pituitary Nanism
D005260 Female Females

Related Publications

S Yokoya, and S Suwa, and H Maesaka, and T Tanaka
[Insulin-like growth factor I (somatomedin-C)].
August 2002, Nihon rinsho. Japanese journal of clinical medicine,
S Yokoya, and S Suwa, and H Maesaka, and T Tanaka
Non-suppressible insulin-like activity and sulphaton activity in serum extracts of normal subjects, acromegalics and pituitary dwarfs.
January 1976, Acta endocrinologica,
S Yokoya, and S Suwa, and H Maesaka, and T Tanaka
Immunoreactive somatomedin-C/insulin-like growth factor I and its binding protein in human milk.
June 1984, The Journal of clinical endocrinology and metabolism,
S Yokoya, and S Suwa, and H Maesaka, and T Tanaka
Growth hormone increases ovarian levels of immunoreactive somatomedin C/insulin-like growth factor I in vivo.
February 1986, Endocrinology,
S Yokoya, and S Suwa, and H Maesaka, and T Tanaka
Somatomedin-C/insulin-like growth factor I in acromegaly.
August 1986, Clinics in endocrinology and metabolism,
S Yokoya, and S Suwa, and H Maesaka, and T Tanaka
[Relationship between immunoreactive insulin and plasma somatomedin-C/insulin-like growth factor-I concentration in childhood obesity].
July 1994, Orvosi hetilap,
S Yokoya, and S Suwa, and H Maesaka, and T Tanaka
Radioimmunoassay of somatomedin C/insulin-like growth factor I.
January 1987, Methods in enzymology,
S Yokoya, and S Suwa, and H Maesaka, and T Tanaka
Purification of somatomedin-C/insulin-like growth factor I.
January 1985, Methods in enzymology,
S Yokoya, and S Suwa, and H Maesaka, and T Tanaka
Erythrocyte insulin-like growth factor-I receptor evaluation in normal subjects, acromegalics, and growth hormone-deficient and insulin-dependent diabetic children.
July 1995, Metabolism: clinical and experimental,
S Yokoya, and S Suwa, and H Maesaka, and T Tanaka
Presence of a insulin-like growth factor I (somatomedin C) immunoreactive substance in GH producing cells in the bovine anterior pituitary.
April 1989, Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme,
Copied contents to your clipboard!