Simultaneous quantification of cefuroxime and clindamycin in human lumbar anulus fibrosus, nucleus pulposus and serum via UPLC-MS/MS. 2021

Xiufang Liang, and Zhongping Gou, and Xiandi Wang, and Yongsheng Wang, and Jiao Yue, and Na Li, and Ping Feng, and Yongping Qin, and Jiancheng Zeng
Institute of Clinical Trials, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, PR China.

BACKGROUND This study aimed to develop a sensitive, accurate method for simultaneously quantifying cefuroxime and clindamycin in human serum, lumbar anulus fibrosus and nucleus pulposus. METHODS Cefuroxime and clindamycin were quantified using ultra high-performance liquid chromatography-electrospray ionization tandem mass spectrometry in multiple-reaction-monitoring mode on a triple-quadrupole AB Qtrap 5500 system in positive ion mode. Internal standards were D3-cefuroxime and D3,13C-clindamycin. Samples were pretreated by precipitating total protein. RESULTS The method showed high sensitivity and good linearity over broad calibration ranges from 100 to 100 000 ng/mL for cefuroxime and 10 to 10 000 ng/mL for clindamycin in serum, and from 10 to 10 000 ng/mL for cefuroxime and 1 to 1 000 ng/mL for clindamycin in lumbar nucleus pulposus. In all sample types, correlation coefficients were greater than 0.99, intra- and inter-day precision (relative standard deviation) was less than 15%, and accuracy (relative error) was within 14% for both analytes. This method was effective at quantifying penetration of cefuroxime and clindamycin in patients undergoing oblique lumbar interbody fusion surgery. CONCLUSIONS A very sensitive, specific method for simultaneous detection of cefuroxime and clindamycin has been developed for human lumbar anulus fibrosus, nucleus pulposus and serum samples.

UI MeSH Term Description Entries
D008161 Lumbosacral Region Region of the back including the LUMBAR VERTEBRAE, SACRUM, and nearby structures. Lumbar Region,Lumbar Regions,Lumbosacral Regions,Region, Lumbar,Region, Lumbosacral,Regions, Lumbar,Regions, Lumbosacral
D002444 Cefuroxime Broad-spectrum cephalosporin antibiotic resistant to beta-lactamase. It has been proposed for infections with gram-negative and gram-positive organisms, GONORRHEA, and HAEMOPHILUS. Cephuroxime,Ketocef,Zinacef
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D002981 Clindamycin An antibacterial agent that is a semisynthetic analog of LINCOMYCIN. 7-Chloro-7-deoxylincomycin,Chlolincocin,Chlorlincocin,Cleocin,Clindamycin Hydrochloride,Clindamycin Monohydrochloride,Clindamycin Monohydrochloride, Monohydrate,Dalacin C,7 Chloro 7 deoxylincomycin,Hydrochloride, Clindamycin,Monohydrate Clindamycin Monohydrochloride,Monohydrochloride, Clindamycin,Monohydrochloride, Monohydrate Clindamycin
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000070614 Nucleus Pulposus Fibrocartilage inner core of the intervertebral disc. Prolapsed or bulged nucleus pulposus leads to INTERVERTEBRAL DISC DISPLACEMENT while proliferation of cells in the nucleus pulposus is associated with INTERVERTEBRAL DISC DEGENERATION.
D000070616 Annulus Fibrosus Outer portion of the intervertebral disc that surrounds the NUCLEUS PULPOSUS. Anulus Fibrosus
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity
D015203 Reproducibility of Results The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results. Reliability and Validity,Reliability of Result,Reproducibility Of Result,Reproducibility of Finding,Validity of Result,Validity of Results,Face Validity,Reliability (Epidemiology),Reliability of Results,Reproducibility of Findings,Test-Retest Reliability,Validity (Epidemiology),Finding Reproducibilities,Finding Reproducibility,Of Result, Reproducibility,Of Results, Reproducibility,Reliabilities, Test-Retest,Reliability, Test-Retest,Result Reliabilities,Result Reliability,Result Validities,Result Validity,Result, Reproducibility Of,Results, Reproducibility Of,Test Retest Reliability,Validity and Reliability,Validity, Face
D016014 Linear Models Statistical models in which the value of a parameter for a given value of a factor is assumed to be equal to a + bx, where a and b are constants. The models predict a linear regression. Linear Regression,Log-Linear Models,Models, Linear,Linear Model,Linear Regressions,Log Linear Models,Log-Linear Model,Model, Linear,Model, Log-Linear,Models, Log-Linear,Regression, Linear,Regressions, Linear

Related Publications

Xiufang Liang, and Zhongping Gou, and Xiandi Wang, and Yongsheng Wang, and Jiao Yue, and Na Li, and Ping Feng, and Yongping Qin, and Jiancheng Zeng
January 1986, Acta anatomica,
Xiufang Liang, and Zhongping Gou, and Xiandi Wang, and Yongsheng Wang, and Jiao Yue, and Na Li, and Ping Feng, and Yongping Qin, and Jiancheng Zeng
August 2007, Spine,
Xiufang Liang, and Zhongping Gou, and Xiandi Wang, and Yongsheng Wang, and Jiao Yue, and Na Li, and Ping Feng, and Yongping Qin, and Jiancheng Zeng
May 2007, Spine,
Xiufang Liang, and Zhongping Gou, and Xiandi Wang, and Yongsheng Wang, and Jiao Yue, and Na Li, and Ping Feng, and Yongping Qin, and Jiancheng Zeng
June 2004, Spine,
Xiufang Liang, and Zhongping Gou, and Xiandi Wang, and Yongsheng Wang, and Jiao Yue, and Na Li, and Ping Feng, and Yongping Qin, and Jiancheng Zeng
January 2009, Spine,
Xiufang Liang, and Zhongping Gou, and Xiandi Wang, and Yongsheng Wang, and Jiao Yue, and Na Li, and Ping Feng, and Yongping Qin, and Jiancheng Zeng
February 1996, Spine,
Xiufang Liang, and Zhongping Gou, and Xiandi Wang, and Yongsheng Wang, and Jiao Yue, and Na Li, and Ping Feng, and Yongping Qin, and Jiancheng Zeng
March 2005, Biomechanics and modeling in mechanobiology,
Xiufang Liang, and Zhongping Gou, and Xiandi Wang, and Yongsheng Wang, and Jiao Yue, and Na Li, and Ping Feng, and Yongping Qin, and Jiancheng Zeng
August 2001, Spine,
Xiufang Liang, and Zhongping Gou, and Xiandi Wang, and Yongsheng Wang, and Jiao Yue, and Na Li, and Ping Feng, and Yongping Qin, and Jiancheng Zeng
June 2009, Spine,
Xiufang Liang, and Zhongping Gou, and Xiandi Wang, and Yongsheng Wang, and Jiao Yue, and Na Li, and Ping Feng, and Yongping Qin, and Jiancheng Zeng
March 2020, Biomedical chromatography : BMC,
Copied contents to your clipboard!