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The authors have recently developed a monoclonal antibody, HHF35, that recognizes the muscle-specific isoforms of actin. To determine its potential usefulness in the differential diagnosis of "small, round, blue cell" tumors of childhood, they immunolabeled formalinor B-5-fixed tissue sections from known cases of rhabdomyosarcoma or rhabdomyoma (30), neuroblastoma (9), retinoblastoma (2), and Ewing's sarcoma (9) with HHF35 and with antibodies to creatine kinase M, myoglobin, vimentin, and neuron-specific enolase. HHF35 reacted with 29 of 30 cases of rhabdomyosarcoma, whereas antibodies to creatine kinase M and myoglobin were positive on only 12 and 7 tumors, respectively. HHF35 did not react with any case of neuroblastoma, retinoblastoma, or Ewing's sarcoma when the antibody diluent contained 50 mM EDTA. These results indicate that HHF35 is a highly sensitive and specific marker for myogenic differentiation and that it will be useful in the differential diagnosis of rhabdomyosarcomas.
R A Schmidt, and
R Cone, and
J E Haas, and
A M Gown
August 1999,
Fa yi xue za zhi,
R A Schmidt, and
R Cone, and
J E Haas, and
A M Gown
January 1987,
The American journal of pathology,
R A Schmidt, and
R Cone, and
J E Haas, and
A M Gown
May 1987,
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R A Schmidt, and
R Cone, and
J E Haas, and
A M Gown
February 1985,
American journal of hematology,
R A Schmidt, and
R Cone, and
J E Haas, and
A M Gown
June 1982,
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R A Schmidt, and
R Cone, and
J E Haas, and
A M Gown
December 1983,
Biochemistry international,
R A Schmidt, and
R Cone, and
J E Haas, and
A M Gown
August 1987,
Biochemical and biophysical research communications,
R A Schmidt, and
R Cone, and
J E Haas, and
A M Gown
January 1994,
Cell biophysics,
R A Schmidt, and
R Cone, and
J E Haas, and
A M Gown
January 1991,
Methods in enzymology,
R A Schmidt, and
R Cone, and
J E Haas, and
A M Gown
January 1992,
Dakar medical,
