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First complete genome sequence of an isolate of cowpea severe mosaic virus from South America. 2021

Kelvin Josemar M L Teixeira, and Renan de Souza Cascardo, and Lorhan L Leal, and F Murilo Zerbini, and José Evando A Beserra
Dep. de Fitotecnia, Universidade Federal do Piauí, Teresina, PI, 64049-550, Brazil.

In this study, the complete nucleotide sequence of a Brazilian isolate of cowpea severe mosaic virus (CPSMV) is presented for the first time. To date, the CPSMV-DG isolate, from the USA, is the only one with the complete known genome. High-throughput sequencing (Illumina HiSeq) and Sanger sequencing of the total RNA extract from a cowpea plant collected in Teresina city, Brazil, revealed the genome sequence of the CPSMV-Ter1 isolate. RNA-1 and RNA-2 are, respectively, 5921 and 3465 nucleotides (nt) long without the poly(A) tail, and show 77.91% and 76.08% nt sequence identity with CPSMV-DG, considered the type isolate of the species. The open reading frames (ORFs) were determined and the cleavage sites of the polyproteins were predicted. Although the two isolates show a similar genomic organization, there was a low percentage of sequence identity between Ter1 and DG. Furthermore, pairwise comparisons of a partial RNA-1 fragment between CPSMV-Ter1 and 11 CPSMV isolates from Brazil indicated 94.6 to 94.8% nt and 98.9% to 99.4% aa sequence identities.

UI MeSH Term Description Entries
D001938 Brazil A country located on the eastern coast of South America, located between Colombia and Peru, that borders the Atlantic Ocean. It is bordered on the north by Venezuela, Guyana, Suriname, and French Guiana, on the south by Uruguay, and on the west by Argentina. The capital is Brasilia.
D000070658 Vigna Plant genus in the pea family (Fabaceae). Adzuki Bean,Black Gram,Blackeyed Pea,Cowpeas,Moth Bean,Mung Beans,Phaseolus mungo,Rice Bean,Vigna aconitifolia,Vigna angularis,Vigna mungo,Vigna radiata,Vigna umbellata,Vigna unguiculata,Adzuki Beans,Bean, Adzuki,Bean, Moth,Bean, Mung,Bean, Rice,Beans, Adzuki,Beans, Moth,Beans, Mung,Beans, Rice,Blackeyed Peas,Cowpea,Gram, Black,Moth Beans,Mung Bean,Pea, Blackeyed,Peas, Blackeyed,Rice Beans
D000073336 Whole Genome Sequencing Techniques to determine the entire sequence of the GENOME of an organism or individual. Complete Genome Sequencing,Genome Sequencing, Complete,Genome Sequencing, Whole,Sequencing, Complete Genome,Sequencing, Whole Genome
D012367 RNA, Viral Ribonucleic acid that makes up the genetic material of viruses. Viral RNA
D016679 Genome, Viral The complete genetic complement contained in a DNA or RNA molecule in a virus. Viral Genome,Genomes, Viral,Viral Genomes
D017423 Sequence Analysis, RNA A multistage process that includes cloning, physical mapping, subcloning, sequencing, and information analysis of an RNA SEQUENCE. RNA Sequence Analysis,Sequence Determination, RNA,Analysis, RNA Sequence,Determination, RNA Sequence,Determinations, RNA Sequence,RNA Sequence Determination,RNA Sequence Determinations,RNA Sequencing,Sequence Determinations, RNA,Analyses, RNA Sequence,RNA Sequence Analyses,Sequence Analyses, RNA,Sequencing, RNA
D017798 Comovirus A genus of plant viruses of the family COMOVIRIDAE in which the bipartite genome is encapsidated in separate icosahedral particles. Mosaic and mottle symptoms are characteristic, and transmission is exclusively by leaf-feeding beetles. Cowpea mosaic virus is the type species. Cowpea Mosaic Virus,Cowpea Mosaic Viruses,Mosaic Virus, Cowpea,Mosaic Viruses, Cowpea

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