We have studied the effect of the length of incubation on the reliability of commercial exoantigen test reagents (Nolan/Scott Biological Laboratories Inc, and Immuno-Mycologics Inc). Antigen extracts, tested in each commercial system, were prepared from duplicate sets of cultures of Histoplasma capsulatum (12 isolates) and of Blastomyces dermatitidis (11 isolates) after one to six weeks of growth. For isolates of H capsulatum, antigens necessary for immunoidentification were detected in most cultures in two weeks and in all cultures after four weeks of incubation for both Nolan and Immuno-Mycologics reagents, and continued to be detected for at least six weeks. Positive results could usually be obtained from exoantigen tests for H capsulatum before dimorphism could be demonstrated by conversion of the mold to yeast. Diagnostically significant antigen was not uniformly present in all cultures of B dermatitidis during the test period, but ten of 11 isolates were positive at some time during the six weeks using the Nolan reagents; antigen of only one of 11 isolates was detected by the Immuno-Mycologic reagents. Results of exoantigen tests for B dermatitidis were usually not available until after the identity had been confirmed by conversion of the mold to yeast phase on cottonseed agar.