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Isolation of the major O-glycosidically linked oligosaccharides obtained by alkaline borohydride degradation of human meconium glycoproteins. 1988
Laboratoire de Chimie Biologique, Unité Associée au CNRS No. 217, Université des Sciences et Techniques de Lille Flandres-Artois, Villeneuve d'Ascq, France.
Neutral and acidic oligosaccharides derived from human meconium glycoproteins by alkaline borohydride degradation have been separated by high-performance liquid chromatography on a Micro-Pak anion-exchange column. In each class, oligosaccharides were purified by normal-phase (neutral and acidic oligosaccharides) and reversed-phase (neutral oligosaccharides) chromatography. Effective separations of neutral oligosaccharides and acidic oligosaccharides were achieved.
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