The objective of the present study was to determine the location of the neurons that give rise to catecholamine-containing terminals in the nucleus tractus solitarii. This was done by injecting rhodamine-filled latex microspheres into the nucleus tractus solitarii of rats to retrogradely label neuronal cell bodies and by processing sections from the brains of these animals to determine if the labelled neurons were immunoreactive for the catecholamine-synthesizing enzymes, dopamine-beta-hydroxylase (DBH) and phenylethanolamine-N-methyl transferase (PNMT). Approximately 60% of the DBH-immunoreactive neurons that projected to the nucleus tractus solitarii belonged to the A1/C1 cell group, while an additional 20% belonged to the A5 cell group. Thus, these two ventrolateral rhombencephalic cell groups accounted for nearly 80% of the total number of rhodamine-bead-labelled DBH-immunoreactive neurons in this series of experiments. Only a small number of DBH-immunoreactive neurons of the A2/C2 cell group contained rhodamine-filled latex microspheres. Rarely, DBH-immunoreactive neurons in the locus coeruleus and the nucleus subcoeruleus were found to project to the nucleus tractus solitarii. The majority of the PNMT-immunoreactive neurons that projected to the nucleus tractus solitarii belonged to the C1 cell group. Only small numbers of PNMT-immunoreactive neurons of the C2 and C3 groups were found to contain rhodamine-filled latex microspheres. It is concluded that neurons in the ventrolateral medulla and pons, some of which presumably utilize norepinephrine and/or epinephrine as a transmitter, could regulate autonomic function via direct projections to the nucleus tractus solitarii.