In vitro and in vivo results obtained from a novel flexible amperometric oxygen sensor are reported. The sensor is fabricated using thin film deposition techniques and is operated by the application of a pulsed waveform. Development of the sensor was undertaken in order to produce a device that is capable of being sited at the interface of a wound and an overlying wound dressing. Oxygen determinations in such an environment would aid in gaining an understanding of the role of oxygen in wound healing and the type of wound dressing that would provide an environment conducive towards wound healing. In vitro data indicate that linearity of response is good although other performance characteristics are irreproducible. In vivo response to oxygen has been observed 50 h after insertion into a porcine sham wound. Expected trends were followed when changes to the oxygen regime of the wound space were effected, but absolute values of oxygen tension are difficult to state with certainty. This may be due to poor calibration stability and inadequate sealing of the sensor from the surrounding environment.