-transparent.png){kind=logo}
An assay using the artificial substrate, 2,4-diamino-10-methyl-pteroylglutamyl-gamma-glutamate (MTX-G1), was developed to measure gamma-glutamyl hydrolase (conjugase), which hydrolyzes folylpolyglutamates. This assay allows us to: 1) measure conjugase for the first time in rat brain and 2) measure conjugase in a reliable, sensitive and inexpensive manner. The MTX-binding assay results were compared to samples analyzed by HPLC and found to vary by only 13%. The artificial substrate, MTX-G1, had a lower rate of hydrolysis than pteroylglutamyl-gamma-glutamate (Pte-G2), 70.7 +/- 0.64 and 92.6 +/- 0.22 nmoles/hr/mg protein respectively. Conjugase was semi-purified 24 fold in H2O and found to have a pH optimum of 5.0.
D M Hartley, and
S R Snodgrass, and
P A Bradshaw
March 1981,
The Journal of nutrition,
D M Hartley, and
S R Snodgrass, and
P A Bradshaw
November 1992,
Analytical biochemistry,
D M Hartley, and
S R Snodgrass, and
P A Bradshaw
August 1975,
The Journal of biological chemistry,
D M Hartley, and
S R Snodgrass, and
P A Bradshaw
March 1988,
The British journal of nutrition,
D M Hartley, and
S R Snodgrass, and
P A Bradshaw
August 1956,
The Journal of biological chemistry,
D M Hartley, and
S R Snodgrass, and
P A Bradshaw
March 1976,
The American journal of clinical nutrition,
D M Hartley, and
S R Snodgrass, and
P A Bradshaw
July 1970,
The American journal of clinical nutrition,
D M Hartley, and
S R Snodgrass, and
P A Bradshaw
April 1992,
Cell biology international reports,
D M Hartley, and
S R Snodgrass, and
P A Bradshaw
August 1991,
Cancer research,
D M Hartley, and
S R Snodgrass, and
P A Bradshaw
January 1985,
Radiobiologiia,
