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We have defined variants (V) resistant to 6-thioguanine (TG) by their ability to divide at least once during a 72-h incubation in a medium containing 0.2 mM TG. We blocked cytokinesis by adding cytochalasin B (CB) after 30 h in culture. The cells that had undergone nuclear division were identified by their content of 2 or more nuclei. The long incubation period allowed slow growing V to be counted. As a result we scored an order of magnitude more V than have been reported in assays using the conventional 30-40 h culture times. In the gamma-ray dose range of 0-0.5 Gy we scored 80 V per 1000 surviving lymphocytes per Gy--a result some two orders of magnitude larger than has been reported previously.
A Norman, and
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K S Iwamoto
May 1982,
Mechanisms of ageing and development,
A Norman, and
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K S Iwamoto
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Cancer research,
A Norman, and
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Mutation research,
A Norman, and
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K S Iwamoto
March 1978,
Journal of chromatography,
A Norman, and
J C Mitchell, and
K S Iwamoto
April 1993,
Mutation research,
A Norman, and
J C Mitchell, and
K S Iwamoto
August 1982,
Mutation research,
A Norman, and
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March 1991,
Mutagenesis,
A Norman, and
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February 1990,
Human genetics,
A Norman, and
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K S Iwamoto
January 1985,
Human genetics,
A Norman, and
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K S Iwamoto
January 1991,
Teratogenesis, carcinogenesis, and mutagenesis,
