Positive empathy is the ability to share and understand the positive emotions of others. In recent years, although positive empathy has received more and more attention, trait positive empathy (TPE)-related spontaneous brain activity during the resting state has not been extensively explored. We used the amplitude of low-frequency fluctuations (ALFFs) and resting-state functional connectivity (RSFC) of the resting-state functional magnetic resonance imaging signal to explore TPE-associated brain regions. We found that higher TPE was associated with higher ALFFs in the right insula and lower ALFFs in the right subgenual cingulate (SGC), right dorsomedial prefrontal cortex (dmPFC), and right precuneus. RSFC analyses showed that higher functional connectivity between the right insula and left parahippocampal gyrus, left inferior parietal lobule and left middle temporal gyrus were related to higher TPE. Moreover, the connection between the right dmPFC and the left medial orbitofrontal cortex, left middle occipital gyrus and left posterior cingulate cortex were positively related to TPE. Meanwhile, the strength of functional connectivity between the right SGC and left supplementary motor area was positively associated with TPE. These findings may indicate that TPE is linked to emotional (especially the experience of more positive emotions and better negative emotion regulation) and self-referential processing.