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A primary monolayer culture system from cockerel hepatocytes was established. The cultures synthesize and secrete proteins that comigrate with authentic serum proteins on polyacrylamide gels and are found in the same relative abundance. Addition of estradiol increased the synthesis of apoprotein B, found in very low density lipoprotein, under all culture conditions. Vitellogenin synthesis could not be induced directly by estradiol. However, when serum was obtained from cockerels injected with estradiol 4 days before blood collection and included in the culture medium, the cultures secreted a protein identified immunologically as vitellogenin by affinity chromatography. Furthermore, addition of growth hormone or prolactin to cultured cockerel hepatocyte monolayers resulted in the synthesis and secretion of a polypeptide that comigrates with authentic vitellogenin on polyacrylamide gels.
K D Boehm, and
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J Ilan
February 1980,
The Journal of cell biology,
K D Boehm, and
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J Ilan
June 2003,
General and comparative endocrinology,
K D Boehm, and
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June 2003,
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology,
K D Boehm, and
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J Ilan
May 1988,
Xenobiotica; the fate of foreign compounds in biological systems,
K D Boehm, and
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J Ilan
December 1985,
NIPH annals,
K D Boehm, and
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J Ilan
October 1998,
The Journal of steroid biochemistry and molecular biology,
K D Boehm, and
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J Ilan
April 1985,
Biochemical and biophysical research communications,
K D Boehm, and
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J Ilan
December 2011,
Archives of toxicology,
K D Boehm, and
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J Ilan
August 2016,
Cytotechnology,
K D Boehm, and
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J Ilan
September 1977,
Endocrinology,
