Biomaterial Database

A routine diagnostic assay for the detection of benzimidazole resistance in parasitic nematodes using tritiated benzimidazole carbamates. 1988

E Lacey, and K L Snowdon

CSIRO Division of Animal Health, McMaster Laboratory, Glebe, N.S.W., Australia.

Resistance to benzimidazoles (BZs) in parasitic nematodes has recently been shown to be due to a reduction in the ability of BZs to bind the structural protein, tubulin, in resistant isolates. Based on these observations the development and standardisation of a routine diagnostic assay has been undertaken by measuring the binding of tritiated mebendazole to crude supernatants of L3 larvae. The assay is rapid, requiring less than 2 h, and is robust, highly reproducible and sensitive to minor changes in the resistance status of parasite populations. Investigation of the routine application and validity of this assay has been documented using 24 isolates of known resistance status from the species Haemonchus contortus, Trichostrongylus colubriformis and Ostertagia circumcincta: In all cases the observed binding and calculated susceptibility factors were in accordance with their respective resistance status.

UI	MeSH Term	Description	Entries
D007814	Larva	Wormlike or grublike stage, following the egg in the life cycle of insects, worms, and other metamorphosing animals.	Maggots,Tadpoles,Larvae,Maggot,Tadpole
D008463	Mebendazole	A benzimidazole that acts by interfering with CARBOHYDRATE METABOLISM and inhibiting polymerization of MICROTUBULES.	Anti-Worm,Bantenol,Banworm,Boots Threadworm Treatment,Lomper,Madicure,Mebendan,Mebenvet,Pripsen Mebendazole,R17635,Sqworm,Sufil,Surfont,Telmin,Vermicol,Vermidil,Vermox,Wormkuur,Anti Worm
D010028	Ostertagia	A genus of parasitic nematodes occurring in the stomach of ruminants.	Ostertagia ostertagi,Ostertagia ostertagus,Ostertagias,ostertagi, Ostertagia
D002498	Centrifugation	Process of using a rotating machine to generate centrifugal force to separate substances of different densities, remove moisture, or simulate gravitational effects. It employs a large motor-driven apparatus with a long arm, at the end of which human and animal subjects, biological specimens, or equipment can be revolved and rotated at various speeds to study gravitational effects. (From Websters, 10th ed; McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
D004351	Drug Resistance	Diminished or failed response of an organism, disease or tissue to the intended effectiveness of a chemical or drug. It should be differentiated from DRUG TOLERANCE which is the progressive diminution of the susceptibility of a human or animal to the effects of a drug, as a result of continued administration.	Resistance, Drug
D006189	Haemonchus	A genus of parasitic nematode worms which infest the duodenum and stomach of domestic and wild herbivores, which ingest it with the grasses (POACEAE) they eat. Infestation of man is accidental.	Hemonchus
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001562	Benzimidazoles	Compounds with a BENZENE fused to IMIDAZOLES.
D014251	Trichostrongyloidea	A superfamily of nematodes. Most are intestinal parasites of ruminants and accidentally in humans. This superfamily includes seven genera: DICTYOCAULUS; HAEMONCHUS; Cooperia, OSTERTAGIA; Nematodirus, TRICHOSTRONGYLUS; and Hyostrongylus.	Trichostrongyloideas
D014254	Trichostrongylus	A genus of parasitic nematodes found in the digestive tract of herbivorous animals. They cause incidental infections in humans from the following species: Trichostrongylus colubriformis, T. orientalis, T. axei, and T. probolurus.