Biomaterial Database

Flavonoid-Decorated Nano-gold for Antimicrobial Therapy Against Gram-negative Bacteria Escherichia coli. 2021

Suvadra Das, and Tanay Pramanik, and Megha Jethwa, and Partha Roy

Basic Science and Humanities Department, University of Engineering and Management, Newtown, Kolkata, India. suvadra.das@uem.edu.in.

Nano-gold (Aunps) have emerged as promising options that exhibit unique features discrete from traditional materials suited for biomedical applications. Aunps were synthesized using flavonoid quercetin (Q) as reducing agent, and resultant nanoparticles were further conjugated with the flavonoid. The resultant nano-system was expected to perform a dual role as antibacterial and as antioxidant agent. Nano-gold surface plasmon peaks were recorded at 560 nm with size around 62 nm and having slim distribution pattern. Spherical particle with smooth surface was observed under TEM and AFM studies. TEM micrographs confirmed a homogeneous particle population of size around 30 nm. Quercetin association to nano-gold was corroborated through FTIR and EDAX analysis. Antioxidant nature of nano-gold prevented rapid oxidation of brilliant cresyl blue dye, in presence of sodium hypochlorite. Antimicrobial action of QuAunp was tested against Gram-negative bacteria Escherichia coli. Nano-gold designed produced a minimum inhibitory concentration of 7.6 μg/ml and minimum bactericidal concentration 10.5 μg/ml against E. coli. Further TEM analysis and membrane permeability studies revealed the impact of QuAunps on bacterial membrane leading to cell damage.

UI	MeSH Term	Description	Entries
D011794	Quercetin	A flavonol widely distributed in plants. It is an antioxidant, like many other phenolic heterocyclic compounds. Glycosylated forms include RUTIN and quercetrin.	3,3',4',5,7-Pentahydroxyflavone,Dikvertin
D004926	Escherichia coli	A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.	Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D006046	Gold	A yellow metallic element with the atomic symbol Au, atomic number 79, and atomic weight 197. It is used in jewelry, goldplating of other metals, as currency, and in dental restoration. Many of its clinical applications, such as ANTIRHEUMATIC AGENTS, are in the form of its salts.
D000890	Anti-Infective Agents	Substances that prevent infectious agents or organisms from spreading or kill infectious agents in order to prevent the spread of infection.	Anti-Infective Agent,Anti-Microbial Agent,Antimicrobial Agent,Microbicide,Microbicides,Anti-Microbial Agents,Antiinfective Agents,Antimicrobial Agents,Agent, Anti-Infective,Agent, Anti-Microbial,Agent, Antimicrobial,Agents, Anti-Infective,Agents, Anti-Microbial,Agents, Antiinfective,Agents, Antimicrobial,Anti Infective Agent,Anti Infective Agents,Anti Microbial Agent,Anti Microbial Agents
D053768	Metal Nanoparticles	Nanoparticles produced from metals whose uses include biosensors, optics, and catalysts. In biomedical applications the particles frequently involve the noble metals, especially gold and silver.	Metal Nanocrystals,Metallic Nanocrystals,Metallic Nanoparticles,Metal Nanocrystal,Metal Nanoparticle,Metallic Nanocrystal,Metallic Nanoparticle,Nanocrystal, Metal,Nanocrystal, Metallic,Nanocrystals, Metal,Nanocrystals, Metallic,Nanoparticle, Metal,Nanoparticle, Metallic,Nanoparticles, Metal,Nanoparticles, Metallic