Experimental duck hepatitis B virus infection: pathology and evolution of hepatic and extrahepatic infection. 1988

J S Freiman, and A R Jilbert, and R J Dixon, and M Holmes, and E J Gowans, and C J Burrell, and E J Wills, and Y E Cossart
Department of Infectious Diseases, University of Sydney, NSW, Australia.

Seventy, 1-day-old ducklings inoculated intraperitoneally with duck hepatitis B virus and 30 controls have been studied over a 2-year period. Infection with duck hepatitis B virus occurred in all inoculated ducks, although this was not associated with clinical morbidity. Duck hepatitis B virus DNA was first detected in liver on Day 3, in pancreatic acinar cells on Day 4, serum on Day 6, splenic red and white pulp on Day 7 and in the renal glomurulus on Day 14, using a combination of dot, Southern blot and in situ hybridization techniques. Peak levels of circulating virus, as determined by DNA polymerase levels, occurred 1 to 4 weeks postinoculation. Mild degrees of portal inflammation were seen in sections of liver tissue in both infected and control ducks. However, moderately severe inflammatory changes were present in 8 of 22 infected birds compared with 0 of 18 controls (p less than 0.025). Appearance of this inflammatory infiltrate 6 weeks postinoculation coincided with a decrease in levels of duck hepatitis B virus DNA in hepatocytes and within the pancreatic acinar cells. At the same time, duck hepatitis B virus DNA became increasingly localized to the splenic germinal centers, and viral DNA was first detected in pancreatic islet cells. No histological changes accompanied the extra-hepatic tissue infection. The sequence and significance of duck hepatitis B virus infection in liver and extra-hepatic tissues is discussed in relation to the pathogenesis of hepatitis B virus infection in man.

UI MeSH Term Description Entries
D007150 Immunohistochemistry Histochemical localization of immunoreactive substances using labeled antibodies as reagents. Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D007668 Kidney Body organ that filters blood for the secretion of URINE and that regulates ion concentrations. Kidneys
D008099 Liver A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances. Livers
D008854 Microscopy, Electron Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen. Electron Microscopy
D009693 Nucleic Acid Hybridization Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503) Genomic Hybridization,Acid Hybridization, Nucleic,Acid Hybridizations, Nucleic,Genomic Hybridizations,Hybridization, Genomic,Hybridization, Nucleic Acid,Hybridizations, Genomic,Hybridizations, Nucleic Acid,Nucleic Acid Hybridizations
D010179 Pancreas A nodular organ in the ABDOMEN that contains a mixture of ENDOCRINE GLANDS and EXOCRINE GLANDS. The small endocrine portion consists of the ISLETS OF LANGERHANS secreting a number of hormones into the blood stream. The large exocrine portion (EXOCRINE PANCREAS) is a compound acinar gland that secretes several digestive enzymes into the pancreatic ductal system that empties into the DUODENUM.
D011201 Poultry Diseases Diseases of birds which are raised as a source of meat or eggs for human consumption and are usually found in barnyards, hatcheries, etc. The concept is differentiated from BIRD DISEASES which is for diseases of birds not considered poultry and usually found in zoos, parks, and the wild. Disease, Poultry,Diseases, Poultry,Poultry Disease
D004279 DNA, Viral Deoxyribonucleic acid that makes up the genetic material of viruses. Viral DNA
D004372 Ducks A water bird in the order Anseriformes (subfamily Anatinae (true ducks)) with a broad blunt bill, short legs, webbed feet, and a waddling gait. Duck
D004769 Enterovirus Infections Diseases caused by ENTEROVIRUS. Infections, Enterovirus,Enterovirus Infection,Infection, Enterovirus

Related Publications

J S Freiman, and A R Jilbert, and R J Dixon, and M Holmes, and E J Gowans, and C J Burrell, and E J Wills, and Y E Cossart
March 1989, Zhonghua bing li xue za zhi = Chinese journal of pathology,
J S Freiman, and A R Jilbert, and R J Dixon, and M Holmes, and E J Gowans, and C J Burrell, and E J Wills, and Y E Cossart
March 1992, Chinese medical journal,
J S Freiman, and A R Jilbert, and R J Dixon, and M Holmes, and E J Gowans, and C J Burrell, and E J Wills, and Y E Cossart
March 1992, Chinese medical journal,
J S Freiman, and A R Jilbert, and R J Dixon, and M Holmes, and E J Gowans, and C J Burrell, and E J Wills, and Y E Cossart
January 1990, Hepatology (Baltimore, Md.),
J S Freiman, and A R Jilbert, and R J Dixon, and M Holmes, and E J Gowans, and C J Burrell, and E J Wills, and Y E Cossart
April 1986, Journal of virology,
J S Freiman, and A R Jilbert, and R J Dixon, and M Holmes, and E J Gowans, and C J Burrell, and E J Wills, and Y E Cossart
December 1983, Virology,
J S Freiman, and A R Jilbert, and R J Dixon, and M Holmes, and E J Gowans, and C J Burrell, and E J Wills, and Y E Cossart
July 1980, Nederlands tijdschrift voor geneeskunde,
J S Freiman, and A R Jilbert, and R J Dixon, and M Holmes, and E J Gowans, and C J Burrell, and E J Wills, and Y E Cossart
March 1979, Israel journal of medical sciences,
J S Freiman, and A R Jilbert, and R J Dixon, and M Holmes, and E J Gowans, and C J Burrell, and E J Wills, and Y E Cossart
February 1991, The Journal of general virology,
J S Freiman, and A R Jilbert, and R J Dixon, and M Holmes, and E J Gowans, and C J Burrell, and E J Wills, and Y E Cossart
August 1990, Virus research,
Copied contents to your clipboard!