Clinical relevance: Since acne vulgaris is a disease of the sebaceous glands, it may have potential effects on the ocular surface and tear homoeostasis, which are essential for good vision. Optometrists should be aware of ocular surface pathologies when prescribing glasses or contact lenses for the visual rehabilitation of these young patients.Background: To evaluate the ocular surface features, meibomian glands, and tear parameters of patients with acne vulgaris.Methods: The right eyes of 70 individuals (34 patients with acne vulgaris, 36 healthy volunteers) were evaluated. The tear break-up time of participants was measured, and the Schirmer test was performed. Then, to determine ocular surface characteristics, samples were taken from the conjunctiva for impression cytology. Finally, the loss rates of the upper and lower eyelid meibomian glands were determined by taking meibography (Sirius, CSO, Florence, Italy).Results: Tear break-up time was significantly lower in the study group compared to the control group (p < 0.001). No statistically significant difference was determined between the groups in respect of Nelson grade in the conjunctival impression cytology (p = 0.141). Grade 3 cytological changes were not observed in either group. The median value of the loss rate in the meibomian glands in the upper eyelid of patients with acne vulgaris was 19.10% (IQR: 18%), while it was 8.75% (IQR: 9.53%) in the control group (p = 0.001). The median value of the loss rate in the meibomian glands in the lower eyelid was 15.70% (IQR: 15.13%) and 7.70% (IQR: 6.53%) in the acne vulgaris and control groups, respectively (p < 0.001).Conclusion: Our study shows that patients with acne vulgaris may have a predisposition to meibomian gland damage and tear instability. Therefore, we consider that a more detailed ophthalmologic examination should be performed in patients with acne vulgaris.