-transparent.png){kind=logo}
The synthetic conjugate of the genotoxic compound 2,4 diaminotoluene (2,4 DAT) with gelatin (2,4 DAT-GEL) was employed to elicit specific antibodies directed against a restricted class of aromatic diamines. Using this immunogen, mouse monoclonal antibodies (MAbs) have been produced. These MAbs have been characterized and used in ELISA to detect 2,4 DAT covalently linked to biopolymers. The MAbs could bind to different synthetic 2,4 DAT-biopolymer adducts as well as to DNA from rats treated in vivo with the aromatic diamine, but they did not react with gelatin or biopolymers alone. The use of these MAbs has been investigated in order to develop a highly sensitive test to detect adducts of this genotoxic compound with nuclear DNA.
S Marini, and
G Citro, and
S Di Cesare, and
R Zito, and
B Giardina
June 2001,
Experimental & molecular medicine,
S Marini, and
G Citro, and
S Di Cesare, and
R Zito, and
B Giardina
January 1993,
Bulletin of the Institute of Maritime and Tropical Medicine in Gdynia,
S Marini, and
G Citro, and
S Di Cesare, and
R Zito, and
B Giardina
January 2005,
Journal of biochemistry and molecular biology,
S Marini, and
G Citro, and
S Di Cesare, and
R Zito, and
B Giardina
December 1984,
Applied and environmental microbiology,
S Marini, and
G Citro, and
S Di Cesare, and
R Zito, and
B Giardina
October 1989,
Immunitat und Infektion,
S Marini, and
G Citro, and
S Di Cesare, and
R Zito, and
B Giardina
January 1984,
Folia biologica,
S Marini, and
G Citro, and
S Di Cesare, and
R Zito, and
B Giardina
October 1998,
Applied biochemistry and biotechnology,
S Marini, and
G Citro, and
S Di Cesare, and
R Zito, and
B Giardina
January 1985,
Hybridoma,
S Marini, and
G Citro, and
S Di Cesare, and
R Zito, and
B Giardina
February 2006,
Hybridoma (2005),
S Marini, and
G Citro, and
S Di Cesare, and
R Zito, and
B Giardina
December 1998,
Biomedical and environmental sciences : BES,
