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An artificial ruthenium-containing β-barrel protein for alkene-alkyne coupling reaction. 2021
Andreas Thiel, and
Daniel F Sauer, and
Ulrich Markel, and
M A Stephanie Mertens, and
Tino Polen, and
Ulrich Schwaneberg, and
Jun Okuda
Institute of Inorganic Chemistry, RWTH Aachen University, Landoltweg 1, 52074 Aachen, Germany. jun.okuda@ac.rwth-aachen.de.
A modified Cp*Ru complex, equipped with a maleimide group, was covalently attached to a cysteine of an engineered variant of Ferric hydroxamate uptake protein component: A (FhuA). This synthetic metalloprotein catalyzed the intermolecular alkene-alkyne coupling of 3-butenol with 5-hexynenitrile. When compared with the protein-free Cp*Ru catalyst, the biohybrid catalyst produced the linear product with higher regioselectivity.
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