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[Bushentongluo recipe (BSTL) attenuates bone destruction by inhibiting NF-κB/RANK/RANKL pathway in collagen-induced arthritis (CIA) rats]. 2021

Hailong Liu, and Gang Wang, and Jia Wang, and Tao Wang, and Jiexiang Tian, and Liqin Wang, and Fang Yang
Department of Rheumatology and Bone Disease, Affiliated Hospital of Gansu University of Traditional Chinese Medicine, Lanzhou 730000, China.

Objective To investigate the therapeutic effect of Bushentongluo recipe (BSTL) on bone destruction and its inhibiting effect on NF-κB/RANK/RANKL pathway in collagen-induced arthritis (CIA) rats. Methods SD rats were randomly divided into blank control group, CIA model group, methotrexate (MTX, 1 mg/kg) group, BSTL 0.5 g/kg and 2 g/kg groups, with 10 rats in each. Except the control group, the other rats were injected subcutaneously with type 2 collagen(Col2) at the base of the tail to establish CIA models. After exposure to MTX or BSTL recipe for consecutive 28 days, the pathological change of joint tissues was examined by HE staining. Immunohistochemistry was used to detect NF-κB p65 expression in synovial tissues. The cytokines and anti-Col2 levels were analyzed by ELISA. Western blotting was used to detect the expression of RANKL, RANK and osteoprotegerin (OPG) proteins. Results Compared with the CIA model group, the rats treated with 2 g/kg BSTL for 28 days had lower paw volume, arthritis index (AI), serum levels of IL-1β, IL-18, TNF-α, anti-Col2-IgG, anti-Col2-IgG2a, RANK and RANKL, and higher level of serum OPG. Besides, Western blotting showed that the expression of NF-κB p65, RANK and RANKL proteins decreased, but the expression of OPG protein increased in BSTL 2 g/kg group. Conclusion BSTL can alleviate the rheumatoid arthritis by down-regulating the expression of NF-κB p65, RANKL, RANK proteins, up-regulating OPG protein, and inhibiting systemic inflammation.

UI MeSH Term Description Entries
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001169 Arthritis, Experimental ARTHRITIS that is induced in experimental animals. Immunological methods and infectious agents can be used to develop experimental arthritis models. These methods include injections of stimulators of the immune response, such as an adjuvant (ADJUVANTS, IMMUNOLOGIC) or COLLAGEN. Adjuvant Arthritis,Arthritis, Adjuvant-Induced,Arthritis, Collagen-Induced,Arthritis, Adjuvant,Collagen Arthritis,Arthritides, Collagen,Arthritis, Collagen,Collagen Arthritides,Collagen-Induced Arthritides,Collagen-Induced Arthritis
D001172 Arthritis, Rheumatoid A chronic systemic disease, primarily of the joints, marked by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Etiology is unknown, but autoimmune mechanisms have been implicated. Rheumatoid Arthritis
D013583 Synovial Membrane The inner membrane of a joint capsule surrounding a freely movable joint. It is loosely attached to the external fibrous capsule and secretes SYNOVIAL FLUID. Synovium,Membrana Synovialis Capsulae Articularis,Membrane, Synovial,Membranes, Synovial,Synovial Membranes
D016328 NF-kappa B Ubiquitous, inducible, nuclear transcriptional activator that binds to enhancer elements in many different cell types and is activated by pathogenic stimuli. The NF-kappa B complex is a heterodimer composed of two DNA-binding subunits: NF-kappa B1 and relA. Immunoglobulin Enhancer-Binding Protein,NF-kappa B Complex,Nuclear Factor kappa B,Transcription Factor NF-kB,kappa B Enhancer Binding Protein,Ig-EBP-1,NF-kB,NF-kappaB,Nuclear Factor-Kappab,Complex, NF-kappa B,Enhancer-Binding Protein, Immunoglobulin,Factor NF-kB, Transcription,Factor-Kappab, Nuclear,Ig EBP 1,Immunoglobulin Enhancer Binding Protein,NF kB,NF kappa B Complex,NF kappaB,NF-kB, Transcription Factor,Nuclear Factor Kappab,Transcription Factor NF kB
D017207 Rats, Sprague-Dawley A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company. Holtzman Rat,Rats, Holtzman,Sprague-Dawley Rat,Rats, Sprague Dawley,Holtzman Rats,Rat, Holtzman,Rat, Sprague-Dawley,Sprague Dawley Rat,Sprague Dawley Rats,Sprague-Dawley Rats
D051381 Rats The common name for the genus Rattus. Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus
D053244 Osteoprotegerin A secreted member of the TNF receptor superfamily that negatively regulates osteoclastogenesis. It is a soluble decoy receptor of RANK LIGAND that inhibits both CELL DIFFERENTIATION and function of OSTEOCLASTS by inhibiting the interaction between RANK LIGAND and RECEPTOR ACTIVATOR OF NUCLEAR FACTOR-KAPPA B. Osteoclastogenesis Inhibitory Factor,Receptors, Tumor Necrosis Factor, Member 11b,Tumor Necrosis Factor Receptor Superfamily, Member 11b,FDCR-1 Protein,Follicular Dendritic Cell-Derived Receptor-1,OCIF Protein,Tumor Necrosis Factor Receptor 11b,FDCR 1 Protein,Follicular Dendritic Cell Derived Receptor 1
D053245 RANK Ligand A transmembrane protein belonging to the tumor necrosis factor superfamily that specifically binds RECEPTOR ACTIVATOR OF NUCLEAR FACTOR-KAPPA B and OSTEOPROTEGERIN. It plays an important role in regulating OSTEOCLAST differentiation and activation. Tumor Necrosis Factor Ligand Superfamily Member 11,CD254 Antigen,OPGL Protein,Osteoclast Differentiation Factor,Osteoprotegerin Ligand,RANKL Protein,Receptor Activator of Nuclear Factor-kappa B Ligand,Receptor Activator of Nuclear Factor-kappaB Ligand,TNF Superfamily, Member 11,TRANCE Protein,Tumor Necrosis Factor-Related Activation-Induced Cytokine,Antigen, CD254,Differentiation Factor, Osteoclast,Receptor Activator of Nuclear Factor kappa B Ligand,Receptor Activator of Nuclear Factor kappaB Ligand,Tumor Necrosis Factor Related Activation Induced Cytokine

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