-transparent.png){kind=logo}
Galactose oxidase is a fungal enzyme which is known to oxidize the C-6 hydroxymethyl of galactose and galactosamine to an aldehyde group. It has been widely used in glycoconjugate research, for example in the labeling of asialoglycoproteins. We have developed a simple affinity purification for galactose oxidase using melibiose-polyacrylamide. This affinity procedure was used to purify the enzyme from ammonium sulfate precipitates of culture filtrates of Dactylium dendroides. The material containing proteases and other contaminants is eluted in the buffer wash. The galactose oxidase is then specifically eluted from the column with buffer containing 0.1 M D-fucose or D-galactose. Using this procedure, the enzyme was also purified from commercial samples of galactose oxidase which contain high proteolytic activity.
F M Kelleher, and
S B Dubbs, and
V P Bhavanandan
January 1982,
Methods in enzymology,
F M Kelleher, and
S B Dubbs, and
V P Bhavanandan
February 1987,
Archives of biochemistry and biophysics,
F M Kelleher, and
S B Dubbs, and
V P Bhavanandan
April 2002,
Journal of chromatography. A,
F M Kelleher, and
S B Dubbs, and
V P Bhavanandan
January 1964,
Hoppe-Seyler's Zeitschrift fur physiologische Chemie,
F M Kelleher, and
S B Dubbs, and
V P Bhavanandan
November 1988,
Archives of biochemistry and biophysics,
F M Kelleher, and
S B Dubbs, and
V P Bhavanandan
April 1992,
The Journal of biological chemistry,
F M Kelleher, and
S B Dubbs, and
V P Bhavanandan
September 1965,
Applied microbiology,
F M Kelleher, and
S B Dubbs, and
V P Bhavanandan
January 1964,
Hoppe-Seyler's Zeitschrift fur physiologische Chemie,
F M Kelleher, and
S B Dubbs, and
V P Bhavanandan
January 2001,
Mycopathologia,
F M Kelleher, and
S B Dubbs, and
V P Bhavanandan
January 1988,
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas,
