In-vitro evaluation of microleakage of bioceramic root-end filling materials: A spectrophotometric study. 2021

Ravi Gupta, and Radhika Kewalramani
Department of Conservative Dentistry, Faculty of Dentistry, Melaka Manipal Medical College (Manipal Campus), Manipal Academy of Higher Education, Manipal, 576104, Karnataka, India.

OBJECTIVE To evaluate the microleakage of newer bioceramic root-end filling materials. METHODS Sixty freshly extracted human single-rooted mandibular premolar teeth were selected for the study. Teeth with fractured root, cracks, anddilacerations were rejected. All teeth were cleaned with ultrasonic scalers. Standard access opening was done and root canal treatment was performed with rotary files followed by obturation. After storing in saline for a week apical 3 ​mm of the root was resected at 900 angles to the long axis of the root. Retro cavity preparation was done with ultrasonic tips. The teeth were divided into four groups of 15 specimens each. Group I - Biodentin, GroupII-Bioaggregate, Group III - MTA Plus, and Group IV - MTA. After the restoration of retro cavities of all the teeth as per manufacture instructions, two coats of nail varnish were applied to leave apical 3 ​mm. All teeth were stored in 2% methylene blue for 72 ​h followed by emersion in 65% nitric acid for the next 72 ​h for Dye extraction. The obtained supernatant solution was then centrifuged and optical density or absorbance was measured with a UV spectrophotometer. RESULTS Microleakage was found to be increasing in this order: Biodentin ​< ​Bioaggregate ​< ​MTA Plus ​< ​MTA. No significant difference was found in the microleakage of Biodentin compared to that of Bioaggregate(p ​> ​0.01). CONCLUSIONS All materials exhibit some amount of microleakage. Biodentin shows the least microleakage among all the bioceramic material groups. Hence, Biodentin and bioaggregate are better material of choice for the retrograde filling to prevent microleakage.

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