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The hypothesis that sorbitol accumulation could contribute to a reduced erythrocyte deformability in diabetes was investigated. Erythrocyte sorbitol and erythrocyte viscosity at high and low shear rates were studied in 20 insulin-dependent diabetic (IDDM) and 20 matched control subjects. An increased erythrocyte sorbitol and an increased low-shear erythrocyte viscosity were found in the IDDM patients, but there was no significant correlation (r = .11, NS) between the parameters. Incubation (3 h, 37 degrees C) in a Krebs buffer containing 33.3 mM glucose resulted in a significant sorbitol accumulation, but erythrocyte viscosity was not affected. Despite this fact, addition of 1 mM statil (ICI 128436) in the 5.5- and 33.3-mM glucose media not only prevented erythrocyte sorbitol accumulation but also improved erythrocyte viscosity in diabetic and control subjects. The effect was more pronounced at the low (approximately 16%) than at the high (approximately 2%) shear rate. The effect on erythrocyte viscosity disappeared by washing the erythrocytes after incubation, although erythrocyte sorbitol remained different. Our results suggest that sorbitol accumulation does not contribute to an increased erythrocyte viscosity in diabetes, and statil shows a positive effect on erythrocyte viscosity independent of its aldose reductase-inhibiting property.
E G Rillaerts, and
J J Vertommen, and
I H De Leeuw
September 1988,
Biochemical pharmacology,
E G Rillaerts, and
J J Vertommen, and
I H De Leeuw
October 1975,
Die Medizinische Welt,
E G Rillaerts, and
J J Vertommen, and
I H De Leeuw
January 1983,
La Ricerca in clinica e in laboratorio,
E G Rillaerts, and
J J Vertommen, and
I H De Leeuw
January 1958,
Montpellier medical,
E G Rillaerts, and
J J Vertommen, and
I H De Leeuw
January 1979,
Biochimica et biophysica acta,
E G Rillaerts, and
J J Vertommen, and
I H De Leeuw
March 1983,
Wiener klinische Wochenschrift,
E G Rillaerts, and
J J Vertommen, and
I H De Leeuw
February 1983,
European journal of clinical investigation,
E G Rillaerts, and
J J Vertommen, and
I H De Leeuw
September 1988,
The International journal of artificial organs,
E G Rillaerts, and
J J Vertommen, and
I H De Leeuw
January 1969,
Bibliotheca haematologica,
E G Rillaerts, and
J J Vertommen, and
I H De Leeuw
March 1981,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
