To investigate the relationship between physical fitness and repeated high-intensity effort (RHIE) ability in elite rugby union players, depending on playing position. Thirty-nine players underwent a fitness testing battery composed of a body composition assessment, upper-body strength (1-repetition maximum bench press and 1-repetition maximum bench row), lower-body strength (6-repetition maximum back squat), and power (countermovement jump, countermovement jump with arms, and 20-m sprint), as well as aerobic fitness (Bronco test) and RHIE tests over a 1-week period. Pearson linear correlations were used to quantify relationships between fitness tests and the RHIE performance outcomes (total sprint time [TST] and percentage decrement [%D]). Thereafter, a stepwise multiple regression model was used to verify the influence of physical fitness measures on RHIE ability. TST was strongly to very strongly associated to body fat (BF, r = .82, P < .01), the 20-m sprint (r = .86, P < .01), countermovement jump (r = -.72, P < .01), and Bronco test (r = .90, P < .01). These fitness outcomes were related to %D, with moderate to strong associations (.82 > ∣r∣ > .54, P < .01). By playing position, similar associations were observed in forwards, but RHIE ability was only related to the 20-m sprint in backs (r = .53, P < .05). The RHIE performance model equations were TST = 13.69 + 0.01 × BF + 0.08 × Bronco + 10.20 × 20 m and %D = -14.34 + 0.11 × BF +0.18 × Bronco - 9.92 × 20 m. These models explain 88.8% and 68.2% of the variance, respectively. Body composition, lower-body power, and aerobic fitness were highly related with RHIE ability. However, backs expressed a different profile than forwards, suggesting that further research with larger sample sizes is needed to better understand the fitness determinants of backs' RHIE ability.