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This report describes a method for determining specifically and sensitively the degradation of the elastin component within complicated extracellular matrices in vitro. Extracellular matrices rich in elastin were metabolically labeled with [3H]lysine during 3 week cultures of smooth muscle cells under ascorbate-free conditions in vitro. Elastin was quantitated on the basis of labeled desmosine/isodesmosine in the matrices as determined by a cation-exchange HPLC program utilizing a Beckman 6300 amino acid analyzer. The net loss of desmosine/isodesmosine during co-culture of human macrophages with the matrices was then used to assay cellular elastin degradation. This method allows for the production of reproducibly labeled matrices and compares favorably with previously described techniques of elastin degradation by live cells in vitro.
H A Chapman, and
M M Ehrhardt, and
G Crombie, and
L K Duffy
June 2014,
The Analyst,
H A Chapman, and
M M Ehrhardt, and
G Crombie, and
L K Duffy
January 2021,
Methods in molecular biology (Clifton, N.J.),
H A Chapman, and
M M Ehrhardt, and
G Crombie, and
L K Duffy
January 1987,
Clinical & experimental metastasis,
H A Chapman, and
M M Ehrhardt, and
G Crombie, and
L K Duffy
January 2001,
Methods in cell biology,
H A Chapman, and
M M Ehrhardt, and
G Crombie, and
L K Duffy
January 2021,
Methods in molecular biology (Clifton, N.J.),
H A Chapman, and
M M Ehrhardt, and
G Crombie, and
L K Duffy
January 2014,
Methods in molecular biology (Clifton, N.J.),
H A Chapman, and
M M Ehrhardt, and
G Crombie, and
L K Duffy
December 1990,
Cell differentiation and development : the official journal of the International Society of Developmental Biologists,
H A Chapman, and
M M Ehrhardt, and
G Crombie, and
L K Duffy
February 2008,
Clinical and experimental immunology,
H A Chapman, and
M M Ehrhardt, and
G Crombie, and
L K Duffy
January 2019,
Matrix biology : journal of the International Society for Matrix Biology,
H A Chapman, and
M M Ehrhardt, and
G Crombie, and
L K Duffy
September 1998,
Pathologie-biologie,
