BIOMAT Database Logo BIOMAT Database Logo

Toxoplasma gondii maintenance in tissue culture: a new efficient method for culturing RH tachyzoites. 1988

F R Noriega, and W E Hauser
Evans Memorial Department for Clinical Research, Boston University Medical Center, Massachusetts 02118.

We describe here a new tissue culture method for prolonged laboratory maintenance of tachyzoites of the highly virulent RH strain of Toxoplasma gondii. Using a rapidly proliferating murine tumor cell line (YAC-1), the method described is easy to perform and is as or more efficient (both in terms of yield and cost) than other traditional methods for maintenance of the parasite. Furthermore, upon prolonged maintenance (greater than 160 days) in YAC-1 tissue culture, the pathogenicity of the parasite, as well as its capacity to elicit an immune response, are comparable to that of organisms maintained in mice. We conclude therefore, that the method described herein is a suitable alternative to the traditional method of maintenance of virulent RH strain T. gondii tachyzoites.

UI MeSH Term Description Entries
D008264 Macrophages The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.) Bone Marrow-Derived Macrophages,Monocyte-Derived Macrophages,Macrophage,Macrophages, Monocyte-Derived,Bone Marrow Derived Macrophages,Bone Marrow-Derived Macrophage,Macrophage, Bone Marrow-Derived,Macrophage, Monocyte-Derived,Macrophages, Bone Marrow-Derived,Macrophages, Monocyte Derived,Monocyte Derived Macrophages,Monocyte-Derived Macrophage
D008722 Methods A series of steps taken in order to conduct research. Techniques,Methodological Studies,Methodological Study,Procedures,Studies, Methodological,Study, Methodological,Method,Procedure,Technique
D003365 Costs and Cost Analysis Absolute, comparative, or differential costs pertaining to services, institutions, resources, etc., or the analysis and study of these costs. Affordability,Analysis, Cost,Cost,Cost Analysis,Cost Comparison,Cost Measures,Cost-Minimization Analysis,Costs and Cost Analyses,Costs, Cost Analysis,Pricing,Affordabilities,Analyses, Cost,Analyses, Cost-Minimization,Analysis, Cost-Minimization,Comparison, Cost,Comparisons, Cost,Cost Analyses,Cost Comparisons,Cost Measure,Cost Minimization Analysis,Cost, Cost Analysis,Cost-Minimization Analyses,Costs,Measure, Cost,Measures, Cost
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D014122 Toxoplasma A genus of protozoa parasitic to birds and mammals. T. gondii is one of the most common infectious pathogenic animal parasites of man. Toxoplasma gondii,Toxoplasma gondius,Toxoplasmas,gondius, Toxoplasma
D014407 Tumor Cells, Cultured Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely. Cultured Tumor Cells,Neoplastic Cells, Cultured,Cultured Neoplastic Cells,Cell, Cultured Neoplastic,Cell, Cultured Tumor,Cells, Cultured Neoplastic,Cells, Cultured Tumor,Cultured Neoplastic Cell,Cultured Tumor Cell,Neoplastic Cell, Cultured,Tumor Cell, Cultured
D051379 Mice The common name for the genus Mus. Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus

Related Publications

F R Noriega, and W E Hauser
Toxoplasma gondii RH Ankara: production of evolving tachyzoites using a novel cell culture method.
May 2011, Experimental parasitology,
F R Noriega, and W E Hauser
[In vitro culture of tachyzoites of Toxoplasma gondii RH strain in HeLa cells].
December 2008, Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases,
F R Noriega, and W E Hauser
Experimental conversion of virulent RH Toxoplasma gondii tachyzoites in vitro.
January 2001, Eastern Mediterranean health journal = La revue de sante de la Mediterranee orientale = al-Majallah al-sihhiyah li-sharq al-mutawassit,
F R Noriega, and W E Hauser
Do Toxoplasma gondii RH strain tachyzoites evolve during continuous passage?
June 2004, Journal of clinical pathology,
F R Noriega, and W E Hauser
Cell-culture system for continuous production of Toxoplasma gondii tachyzoites.
December 1999, European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology,
F R Noriega, and W E Hauser
[Cryopreservation of Toxoplasma gondii tachyzoites and tissue cysts].
January 2013, Turkiye parazitolojii dergisi,
F R Noriega, and W E Hauser
[Characteristics of Toxoplasma-gondii-tachyzoites from different culture systems]
January 1998, ALTEX,
F R Noriega, and W E Hauser
Acidocalcisomes in Toxoplasma gondii tachyzoites.
January 1996, The Biochemical journal,
F R Noriega, and W E Hauser
Toxoplasma gondii in tissue culture and maintenance at low temperatures.
January 1968, Zeitschrift fur Parasitenkunde (Berlin, Germany),
F R Noriega, and W E Hauser
Comparison of Eight Cell-Free Media for Maintenance of Toxoplasma gondii Tachyzoites.
January 2016, Iranian journal of parasitology,
Copied contents to your clipboard!