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We determined the effect of 5,5'-diphenylhydantoin (DPH) on the kinetics of T3 and T4 uptake in cultured GH-producing (GC) cells under serum-free conditions. GC cells accumulated [125I]T3 at a greater fractional rate than [125I]T4. The t 1/2 of exit of [125I]T4 and [125I]T3 previously equilibrated in GC cells was 28 min for T4 and 66 min for T3. T3 and T4 entry rates were not influenced by up to a 10,000-fold molar excess of nonradioactive T3, T4, d-T4, rT3, 3,5-T2 and diiodotyrosine. Thus, entry of T3 and T4 in GC cells appeared nonsaturable and was not influenced by various thyroid hormone analogues. DPH, 25-200 mumol/l, decreased the rate of T3 entry in a dose-dependent manner and did not influence the T3 exit rate. At 200 mumol/l DPH, T3 entry decreased by 40%. Rates of entry and exit of T4 were unaffected by DPH. DPH may affect T3 and T4 entry differentially at the level of the plasma membrane.
L R Zemel, and
D R Biezunski, and
L E Shapiro, and
M I Surks
June 1985,
Endocrinology,
L R Zemel, and
D R Biezunski, and
L E Shapiro, and
M I Surks
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L R Zemel, and
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M I Surks
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L R Zemel, and
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M I Surks
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L R Zemel, and
D R Biezunski, and
L E Shapiro, and
M I Surks
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Endocrinology,
L R Zemel, and
D R Biezunski, and
L E Shapiro, and
M I Surks
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Endocrinology,
L R Zemel, and
D R Biezunski, and
L E Shapiro, and
M I Surks
May 1971,
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L R Zemel, and
D R Biezunski, and
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M I Surks
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L R Zemel, and
D R Biezunski, and
L E Shapiro, and
M I Surks
February 1977,
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L R Zemel, and
D R Biezunski, and
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M I Surks
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