-transparent.png){kind=logo}
We developed a novel chemical synthesis of thiamine triphosphate which allows us to incorporate 32P in the gamma position. The reaction is based on the condensation of [32P]orthophosphoric acid and thiamine diphosphate in the presence of ethyl chloroformate. After purification by two ion-exchange purification steps, the thiamine derivative has a specific radioactivity of 10 Ci/mmol. The average final yield synthesis is about 10%.
Related Publications
C Grandfils, and
L Bettendorff, and
C de Rycker, and
E Schoffeniels
December 1973,
Biochimica et biophysica acta,
C Grandfils, and
L Bettendorff, and
C de Rycker, and
E Schoffeniels
November 2003,
Analytical biochemistry,
C Grandfils, and
L Bettendorff, and
C de Rycker, and
E Schoffeniels
December 1973,
The Journal of biological chemistry,
C Grandfils, and
L Bettendorff, and
C de Rycker, and
E Schoffeniels
November 1987,
FEBS letters,
C Grandfils, and
L Bettendorff, and
C de Rycker, and
E Schoffeniels
January 1978,
Preparative biochemistry,
C Grandfils, and
L Bettendorff, and
C de Rycker, and
E Schoffeniels
October 1964,
Biochimica et biophysica acta,
C Grandfils, and
L Bettendorff, and
C de Rycker, and
E Schoffeniels
June 1985,
Neurochemical research,
C Grandfils, and
L Bettendorff, and
C de Rycker, and
E Schoffeniels
September 1985,
Analytical biochemistry,
C Grandfils, and
L Bettendorff, and
C de Rycker, and
E Schoffeniels
January 1955,
Bollettino della Societa italiana di biologia sperimentale,
C Grandfils, and
L Bettendorff, and
C de Rycker, and
E Schoffeniels
January 1979,
Methods in enzymology,
