BIOMAT Database Logo BIOMAT Database Logo

Blood glucose, lactate, pyruvate, glycerol, 3-hydroxybutyrate and acetoacetate measurements in man using a centrifugal analyser with a fluorimetric attachment. 1988

J Harrison, and A W Hodson, and A W Skillen, and R Stappenbeck, and L Agius, and K G Alberti
Department of Clinical Biochemistry and Metabolic Medicine, Medical School, Newcastle upon Tyne, UK.

Methods are described for the analysis of glucose, lactate, pyruvate, alanine, glycerol, 3-hydroxybutyrate and acetoacetate in perchloric acid extracts of human blood, using the Cobas Bio centrifugal analyser fitted with a fluorimetric attachment. Intra-assay and inter-assay coefficients of variation ranged from 1.9 to 7.9% and from 1.0 to 7.2% respectively. Correlation coefficients ranged from 0.96 to 0.99 against established continuous-flow and manual spectrophotometric methods. All seven metabolites can be measured using a single perchloric acid extract of 20 microliter of blood. The versatility of the assays is such that as little as 100 pmol pyruvate, 3-hydroxybutyrate or as much as 15 nmol glucose can be measured in the same 20 microliter extract.

UI MeSH Term Description Entries
D007773 Lactates Salts or esters of LACTIC ACID containing the general formula CH3CHOHCOOR.
D011773 Pyruvates Derivatives of PYRUVIC ACID, including its salts and esters.
D001786 Blood Glucose Glucose in blood. Blood Sugar,Glucose, Blood,Sugar, Blood
D002498 Centrifugation Process of using a rotating machine to generate centrifugal force to separate substances of different densities, remove moisture, or simulate gravitational effects. It employs a large motor-driven apparatus with a long arm, at the end of which human and animal subjects, biological specimens, or equipment can be revolved and rotated at various speeds to study gravitational effects. (From Websters, 10th ed; McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
D005470 Fluorometry An analytical method for detecting and measuring FLUORESCENCE in compounds or targets such as cells, proteins, or nucleotides, or targets previously labeled with FLUORESCENCE AGENTS. Fluorimetry,Fluorometric Analysis,Analysis, Fluorometric
D005990 Glycerol A trihydroxy sugar alcohol that is an intermediate in carbohydrate and lipid metabolism. It is used as a solvent, emollient, pharmaceutical agent, or sweetening agent. 1,2,3-Propanetriol,Glycerin,1,2,3-Trihydroxypropane,Glycerine
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006885 Hydroxybutyrates Salts and esters of hydroxybutyric acid. Hydroxybutyric Acid Derivatives,Hydroxybutyric Acids,Acid Derivatives, Hydroxybutyric
D000090 Acetoacetates Salts and derivatives of acetoacetic acid.

Related Publications

J Harrison, and A W Hodson, and A W Skillen, and R Stappenbeck, and L Agius, and K G Alberti
Optimized methods to measure acetoacetate, 3-hydroxybutyrate, glycerol, alanine, pyruvate, lactate and glucose in human blood using a centrifugal analyser with a fluorimetric attachment.
January 1990, The Journal of automatic chemistry,
J Harrison, and A W Hodson, and A W Skillen, and R Stappenbeck, and L Agius, and K G Alberti
Determination of lactate, pyruvate, beta-hydroxybutyrate and acetoacetate with a centrifugal analyser.
August 1995, European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies,
J Harrison, and A W Hodson, and A W Skillen, and R Stappenbeck, and L Agius, and K G Alberti
Fluorimetric methods for the measurement of intermediate metabolites (lactate, pyruvate, alanine, beta-hydroxybutyrate, glycerol) using a COBAS FARA centrifugal analyser.
January 1993, The Journal of automatic chemistry,
J Harrison, and A W Hodson, and A W Skillen, and R Stappenbeck, and L Agius, and K G Alberti
Direct assays of lactate, pyruvate, beta-hydroxybutyrate, and acetoacetate with a centrifugal analyzer.
March 1978, Clinical chemistry,
J Harrison, and A W Hodson, and A W Skillen, and R Stappenbeck, and L Agius, and K G Alberti
An enzymatic fluorimetric micromethod for the determination of acetoacetate, -hydroxybutyrate, pyruvate and lactate.
July 1971, Clinica chimica acta; international journal of clinical chemistry,
J Harrison, and A W Hodson, and A W Skillen, and R Stappenbeck, and L Agius, and K G Alberti
Perchloric acid interference in enzymatic-fluorimetric-continuous-flow assay methods for measuring glucose, lactate, pyruvate, alanine, glycerol, and 3-hydroxybutyrate in blood.
June 1986, Clinical chemistry,
J Harrison, and A W Hodson, and A W Skillen, and R Stappenbeck, and L Agius, and K G Alberti
Enzymic fluorometric continuous-flow assays for blood glucose, lactate, pyruvate, alanine, glycerol, and 3-hydroxybutyrate.
October 1978, Clinical chemistry,
J Harrison, and A W Hodson, and A W Skillen, and R Stappenbeck, and L Agius, and K G Alberti
[Blood acetoacetate, 3-hydroxybutyrate, lactate, pyruvate and alpha-ketoglutarate behavior in chronic uremia during a single hemodialysis].
November 1969, Giornale di clinica medica,
J Harrison, and A W Hodson, and A W Skillen, and R Stappenbeck, and L Agius, and K G Alberti
A semiautomated fluorimetric method for the enzymatic determination of pyruvate, lactate, acetoacetate, and beta-hydroxybutyrate levels in plasma.
August 1966, The Journal of laboratory and clinical medicine,
J Harrison, and A W Hodson, and A W Skillen, and R Stappenbeck, and L Agius, and K G Alberti
Determination of pyruvate, lactate, acetoacetate, and 3-hydroxybutyrate in serum by capillary isotachophoresis.
October 1981, Journal of chromatography,
Copied contents to your clipboard!