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Flurazepam and three of its metabolic products (desalkyl, hydroxyethyl, and aldehyde metabolites) can be simultaneously quantitated without derivatization by gas chromatography with electron capture detection. After addition of a suitable internal standard, unknown biological samples and calibration standards are extracted at neutral pH into benzene/isoamyl alcohol. The reconstituted extract is chromatographed at 275 degrees C with a 10% OV-101 liquid phase, which allows resolution of all 5 compounds. In some cases a 1% OV-225 liquid phase is used for quantitation of hydroxyethylflurazepam. The method is sufficiently sensitive and reproducible for use in clinical and experimental pharmacokinetic studies.
E S Burstein, and
H Friedman, and
D J Greenblatt, and
A Locniskar, and
H R Ochs
January 1979,
Journal of forensic sciences,
E S Burstein, and
H Friedman, and
D J Greenblatt, and
A Locniskar, and
H R Ochs
March 1981,
Journal of chromatography,
E S Burstein, and
H Friedman, and
D J Greenblatt, and
A Locniskar, and
H R Ochs
March 1978,
Journal - Association of Official Analytical Chemists,
E S Burstein, and
H Friedman, and
D J Greenblatt, and
A Locniskar, and
H R Ochs
January 1983,
Psychopharmacology,
E S Burstein, and
H Friedman, and
D J Greenblatt, and
A Locniskar, and
H R Ochs
November 1974,
Journal of chromatography,
E S Burstein, and
H Friedman, and
D J Greenblatt, and
A Locniskar, and
H R Ochs
September 1982,
Journal of chromatography,
E S Burstein, and
H Friedman, and
D J Greenblatt, and
A Locniskar, and
H R Ochs
July 1980,
Journal of chromatography,
E S Burstein, and
H Friedman, and
D J Greenblatt, and
A Locniskar, and
H R Ochs
September 1977,
Clinical chemistry,
E S Burstein, and
H Friedman, and
D J Greenblatt, and
A Locniskar, and
H R Ochs
July 1980,
Journal of chromatography,
E S Burstein, and
H Friedman, and
D J Greenblatt, and
A Locniskar, and
H R Ochs
March 1978,
Journal - Association of Official Analytical Chemists,
