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A new method for the continuous recording of catecholamine (CA) secretion from the adrenal gland in rats in response to splanchnic nerve stimulation was developed. The method consists of in situ perfusion of the adrenal gland and direct continuous recording of released total CA by an electrochemical detector (ECD). Splanchnic nerve stimulation evoked current responses in a stimulus frequency dependent manner. The amounts of total CA released was linearly related to peak amplitude and to the total integrated output current for various current responses, indicating that the ECD current response in this system represents a highly reliable parameter of the secretory process in the adrenal gland. This system has the advantage of directly and continuously monitoring total CA secretion from the adrenal gland in situ.
K Kumakura, and
A Sato, and
H Suzuki
June 1982,
The Journal of physiology,
K Kumakura, and
A Sato, and
H Suzuki
July 1974,
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K Kumakura, and
A Sato, and
H Suzuki
September 1992,
Clinical and experimental pharmacology & physiology,
K Kumakura, and
A Sato, and
H Suzuki
May 1984,
Neuroscience,
K Kumakura, and
A Sato, and
H Suzuki
September 1995,
Biological & pharmaceutical bulletin,
K Kumakura, and
A Sato, and
H Suzuki
August 1992,
The American journal of physiology,
K Kumakura, and
A Sato, and
H Suzuki
July 1967,
Nature,
K Kumakura, and
A Sato, and
H Suzuki
January 1961,
The Journal of physiology,
K Kumakura, and
A Sato, and
H Suzuki
January 2000,
Clinical and experimental hypertension (New York, N.Y. : 1993),
K Kumakura, and
A Sato, and
H Suzuki
June 1988,
The Journal of physiology,
