During the last century, national public health institutes emerged to address enduring and emergency public health challenges. Previous outbreaks often compelled countries to establish national institutes of public health. Despite historic legacies and contributions to public health, no review of this literature has been published. The aim of this scoping review is to provide an overview of this literature and map characteristics including format, authorship, geographic focus, methods, language, focal topic and public health capacity building domains. The scoping review was guided the Arksey and O'Malley methodological framework and utilised the PRISMA-ScR checklist. A systematic search of Medline OVID and Scopus databases yielded 5731 records. In total, 43 articles met the eligibility criteria. Articles were published in English, Spanish, French and Russian and included perspectives from over 20 countries in Africa, Europe, North America and South America. Three reported methods or collected primary data. Findings reveal a longstanding international interest in leveraging national institutes to address complex public health challenges. Lack of studies reporting methods reveals the need for future research utilising robust methodology. Several articles recommend investment in national public health institutes as a strategy to respond to crises and strengthen countries' public health systems.