Biomaterial Database

Detection of drug-dependent platelet antibodies using immobilized Staphylococcal protein A. 1988

D J Christie, and S S Lennon

Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis.

Serum samples from eight patients suspected of having drug-induced immunologic thrombocytopenia provoked by a variety of medications were tested for drug-dependent platelet antibodies with a newly developed assay that forms rosettes of antibody-coated platelets around Staphylococcal protein A-Sepharose beads. The same samples were then tested with immunofluorescence and 51Cr-release assays. Seven of eight patients (87.5%) tested positive for drug-dependent antibodies by the rosette assay. In contrast, two of eight (25.0%) and one of eight (12.5%) patients tested positive for drug-dependent antibodies by immunofluorescence and 51Cr release, respectively. These results demonstrate that the new rosette method is significantly more sensitive and specific for the detection of drug-dependent platelet antibodies than either immunofluorescence or 51Cr release.

UI	MeSH Term	Description	Entries
D001792	Blood Platelets	Non-nucleated disk-shaped cells formed in the megakaryocyte and found in the blood of all mammals. They are mainly involved in blood coagulation.	Platelets,Thrombocytes,Blood Platelet,Platelet,Platelet, Blood,Platelets, Blood,Thrombocyte
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000906	Antibodies	Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
D012397	Rosette Formation	The in vitro formation of clusters consisting of a cell (usually a lymphocyte) surrounded by antigenic cells or antigen-bearing particles (usually erythrocytes, which may or may not be coated with antibody or antibody and complement). The rosette-forming cell may be an antibody-forming cell, a memory cell, a T-cell, a cell bearing surface cytophilic antibodies, or a monocyte possessing Fc receptors. Rosette formation can be used to identify specific populations of these cells.	Immunocytoadherence,Formation, Rosette,Formations, Rosette,Immunocytoadherences,Rosette Formations
D013205	Staphylococcal Protein A	A protein present in the cell wall of most Staphylococcus aureus strains. The protein selectively binds to the Fc region of human normal and myeloma-derived IMMUNOGLOBULIN G. It elicits antibody activity and may cause hypersensitivity reactions due to histamine release; has also been used as cell surface antigen marker and in the clinical assessment of B lymphocyte function.	Protein A,Protein A, Staphylococcal
D013921	Thrombocytopenia	A subnormal level of BLOOD PLATELETS.	Thrombopenia,Thrombocytopenias,Thrombopenias
D064420	Drug-Related Side Effects and Adverse Reactions	Disorders that result from the intended use of PHARMACEUTICAL PREPARATIONS. Included in this heading are a broad variety of chemically-induced adverse conditions due to toxicity, DRUG INTERACTIONS, and metabolic effects of pharmaceuticals.	Drug-Related Side Effects and Adverse Reaction,Adverse Drug Event,Adverse Drug Reaction,Drug Side Effects,Drug Toxicity,Side Effects of Drugs,Toxicity, Drug,Adverse Drug Events,Adverse Drug Reactions,Drug Event, Adverse,Drug Events, Adverse,Drug Reaction, Adverse,Drug Reactions, Adverse,Drug Related Side Effects and Adverse Reaction,Drug Related Side Effects and Adverse Reactions,Drug Side Effect,Drug Toxicities,Effects, Drug Side,Reactions, Adverse Drug,Side Effect, Drug,Side Effects, Drug,Toxicities, Drug