Expression patterns of peroxiredoxin genes in bronchial epithelial cells exposed to diesel exhaust particles. 2021

Robson Seriani, and Carla Peres de Paula, and Anderson Ferreira da Cunha, and Marcos Antonio de Oliveira, and Paloma Gava Krempel, and Daniela Perroni Frias, and Elnara Marcia Negri, and Thais Mauad, and Mariangela Macchione
Laboratory of Experimental Air Pollution (LIM05), Department of Pathology, School of Medicine, University of São Paulo, FMUSP- Av. Dr. Arnaldo 455, 1°floor, room 1150, São Paulo, SP 01246-903, Brazil; PROSCED - Consulting, Training and Development, Rua Alto Belo, 742, room 01, São Paulo, SP 03478-040, Brazil. Electronic address: prof.robsonseriani@gmail.com.

Several mechanisms have been suggested to explain the adverse effects of air pollutants on airway cells. One such explanation is the presence of high concentrations of oxidants and pro-oxidants in environmental pollutants. All animal and plant cells have developed several mechanisms to prevent damage by oxidative molecules. Among these, the peroxiredoxins (PRDXs) are of interest due to a high reactivity with reactive oxygen species (ROS) through the functioning of the thioredoxin/thioredoxin reductase system. This study aimed to verify the gene expression patterns of the PRDX family in bronchial epithelial airway cells (BEAS-2B) cells exposed to diesel exhaust particles (DEPs) at a concentration of 15 μg/mL for 1 or 2 h because this it is a major component of particulate matter in the atmosphere. There was a significant decrease in mRNA fold changes of PRDX2 (0.43 ± 0.34; *p = 0.0220), PRDX5 (0.43 ± 0.34; *p = 0.0220), and PRDX6 (0.33 ± 0.25; *p = 0.0069) after 1 h of exposure to DEPs. The reduction in mRNA levels may consequently lead to a decrease in the levels of PRDX proteins, increasing oxidative stress in bronchial epithelial cells BEAS-2B and thus, negatively affecting cellular functions.

UI MeSH Term Description Entries
D001980 Bronchi The larger air passages of the lungs arising from the terminal bifurcation of the TRACHEA. They include the largest two primary bronchi which branch out into secondary bronchi, and tertiary bronchi which extend into BRONCHIOLES and PULMONARY ALVEOLI. Primary Bronchi,Primary Bronchus,Secondary Bronchi,Secondary Bronchus,Tertiary Bronchi,Tertiary Bronchus,Bronchi, Primary,Bronchi, Secondary,Bronchi, Tertiary,Bronchus,Bronchus, Primary,Bronchus, Secondary,Bronchus, Tertiary
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D004847 Epithelial Cells Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells. Adenomatous Epithelial Cells,Columnar Glandular Epithelial Cells,Cuboidal Glandular Epithelial Cells,Glandular Epithelial Cells,Squamous Cells,Squamous Epithelial Cells,Transitional Epithelial Cells,Adenomatous Epithelial Cell,Cell, Adenomatous Epithelial,Cell, Epithelial,Cell, Glandular Epithelial,Cell, Squamous,Cell, Squamous Epithelial,Cell, Transitional Epithelial,Cells, Adenomatous Epithelial,Cells, Epithelial,Cells, Glandular Epithelial,Cells, Squamous,Cells, Squamous Epithelial,Cells, Transitional Epithelial,Epithelial Cell,Epithelial Cell, Adenomatous,Epithelial Cell, Glandular,Epithelial Cell, Squamous,Epithelial Cell, Transitional,Epithelial Cells, Adenomatous,Epithelial Cells, Glandular,Epithelial Cells, Squamous,Epithelial Cells, Transitional,Glandular Epithelial Cell,Squamous Cell,Squamous Epithelial Cell,Transitional Epithelial Cell
D005786 Gene Expression Regulation Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation. Gene Action Regulation,Regulation of Gene Expression,Expression Regulation, Gene,Regulation, Gene Action,Regulation, Gene Expression
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001335 Vehicle Emissions Gases, fumes, vapors, and ODORANTS escaping from the cylinders of a gasoline or diesel internal-combustion engine. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed & Random House Unabridged Dictionary, 2d ed) Automobile Exhaust,Diesel Exhaust,Engine Exhaust,Vehicle Emission,Vehicular Emission,Traffic-Related Pollutants,Transportation Emissions,Vehicular Emissions,Emission, Vehicle,Emission, Vehicular,Emissions, Transportation,Emissions, Vehicle,Emissions, Vehicular,Exhaust, Automobile,Exhaust, Diesel,Exhaust, Engine,Pollutants, Traffic-Related,Traffic Related Pollutants
D052638 Particulate Matter Particles of any solid substance, generally under 30 microns in size, often noted as PM30. There is special concern with PM1 which can get down to PULMONARY ALVEOLI and induce MACROPHAGE ACTIVATION and PHAGOCYTOSIS leading to FOREIGN BODY REACTION and LUNG DISEASES. Ultrafine Fiber,Ultrafine Fibers,Ultrafine Particle,Ultrafine Particles,Ultrafine Particulate Matter,Air Pollutants, Particulate,Airborne Particulate Matter,Ambient Particulate Matter,Fiber, Ultrafine,Particle, Ultrafine,Particles, Ultrafine,Particulate Air Pollutants,Particulate Matter, Airborne,Particulate Matter, Ambient,Particulate Matter, Ultrafine
D054464 Peroxiredoxins A family of ubiquitously-expressed peroxidases that play a role in the reduction of a broad spectrum of PEROXIDES like HYDROGEN PEROXIDE; LIPID PEROXIDES and peroxinitrite. They are found in a wide range of organisms, such as BACTERIA; PLANTS; and MAMMALS. The enzyme requires the presence of a thiol-containing intermediate such as THIOREDOXIN as a reducing cofactor. Peroxiredoxin,Alkyl Hydroperoxide Reductase,Alkyl Hydroperoxide Reductase C,Alkyl Hydroperoxide Reductase D,Alkylhydroperoxide Reductase,Peroxidoxin,Peroxiredoxin 1,Peroxiredoxin 2,Peroxiredoxin 4,Peroxiredoxin 5,Peroxiredoxin I,Peroxiredoxin II,Peroxiredoxin IV,Peroxiredoxin V,Peroxiredoxin-4,Protector Protein (Mixed-Function Oxidase Systems),Thiol-Specific Antioxidant Protein,Thioredoxin Peroxidase,Thioredoxin Peroxidase 1,Thioredoxin Peroxidase 2,Thioredoxin Peroxidase PMP20,Thioredoxin-Dependent Peroxide Reductase 1,Thioredoxin-Dependent Peroxide Reductase 2,Hydroperoxide Reductase, Alkyl,Thiol Specific Antioxidant Protein,Thioredoxin Dependent Peroxide Reductase 1,Thioredoxin Dependent Peroxide Reductase 2

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