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We report a modification of the technique of Mahoney et al. (Blood 1982; 59: 439) for the determination of sodium-22 (22Na+) uptake in human erythrocytes. This modification facilitates the separation of 22Na+ taken up by erythrocytes from the free 22Na+ in the buffer by the addition of dibutyl phthalate, which forms an immiscible layer between the two. To further improve the sensitivity of 22Na+ uptake, we incubated a range of known numbers of erythrocytes with 22Na+ as opposed to the single cell suspension of known hematocrit used in Mahoney's et al. procedure (1). Erythrocytes are incubated in KCI buffer containing 2627 Bq (0.071 microCi) 22Na+ in a total volume of 0.5 mL for 0.5 h at 37 degrees C. Incubation is terminated by placing the tubes in ice for 10 min and the amount of 22Na+ taken up by the erythrocytes determined. We observe a linear relationship between erythrocyte concentrations (0.5 to 2.5 X 10(9) cells/mL) and percent uptake of 22Na+ (0.37 +/- 0.06 (1 SD) to 1.85 +/- 0.27 (1 SD) of the total 22Na+, respectively). The procedure is simple and sensitive, and can be used in clinical laboratories for the routine evaluation of 22Na+ uptake in erythrocytes.
K K Gambhir, and
R Parui, and
S G Nerurkar, and
N Dave, and
J Mathews, and
P P Mehrotra, and
C L Curry
January 1991,
Comparative biochemistry and physiology. A, Comparative physiology,
K K Gambhir, and
R Parui, and
S G Nerurkar, and
N Dave, and
J Mathews, and
P P Mehrotra, and
C L Curry
August 1982,
Proceedings of the Royal Society of London. Series B, Biological sciences,
K K Gambhir, and
R Parui, and
S G Nerurkar, and
N Dave, and
J Mathews, and
P P Mehrotra, and
C L Curry
March 1971,
The Journal of general physiology,
K K Gambhir, and
R Parui, and
S G Nerurkar, and
N Dave, and
J Mathews, and
P P Mehrotra, and
C L Curry
April 1980,
Life sciences,
K K Gambhir, and
R Parui, and
S G Nerurkar, and
N Dave, and
J Mathews, and
P P Mehrotra, and
C L Curry
March 1973,
The Journal of clinical investigation,
K K Gambhir, and
R Parui, and
S G Nerurkar, and
N Dave, and
J Mathews, and
P P Mehrotra, and
C L Curry
September 1973,
Canadian journal of physiology and pharmacology,
K K Gambhir, and
R Parui, and
S G Nerurkar, and
N Dave, and
J Mathews, and
P P Mehrotra, and
C L Curry
April 1972,
The American journal of physiology,
K K Gambhir, and
R Parui, and
S G Nerurkar, and
N Dave, and
J Mathews, and
P P Mehrotra, and
C L Curry
January 1980,
Biofizika,
K K Gambhir, and
R Parui, and
S G Nerurkar, and
N Dave, and
J Mathews, and
P P Mehrotra, and
C L Curry
August 1976,
The Journal of biological chemistry,
K K Gambhir, and
R Parui, and
S G Nerurkar, and
N Dave, and
J Mathews, and
P P Mehrotra, and
C L Curry
January 1982,
Biofizika,
