Biomaterial Database

Potassium permanganate used to eliminate interference with the Porter-Silber reaction in the butanol extraction method for determination of urinary 17-hydroxycorticosteroids. 1988

T Maeda, and H Matsuzaki, and T Sekine

Central Clinical Laboratories, Tokyo Metropolitan Police Hospital, Japan.

We describe a simple, rapid, and less interference-susceptible method for using the Porter-Silber reaction for determination of urinary 17-hydroxycorticosteroids. The procedure is based on butanol extraction of the steroids after the urine sample is treated with potassium permanganate and sodium bisulfite. These treatments, together with an additional acid- and alkali-washing of the extract, could eliminate most of the substances in urine that interfere with the Porter-Silber reactions. Values so obtained correlated well (r = 0.95) with those by Furuya's method in which beta-glucuronidase is used. Our method may be useful and suitable for a screening test of adrenocortical and pituitary functions.

UI	MeSH Term	Description	Entries
D008722	Methods	A series of steps taken in order to conduct research.	Techniques,Methodological Studies,Methodological Study,Procedures,Studies, Methodological,Study, Methodological,Method,Procedure,Technique
D010084	Oxidation-Reduction	A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).	Redox,Oxidation Reduction
D010900	Pituitary Diseases	Disorders involving either the ADENOHYPOPHYSIS or the NEUROHYPOPHYSIS. These diseases usually manifest as hypersecretion or hyposecretion of PITUITARY HORMONES. Neoplastic pituitary masses can also cause compression of the OPTIC CHIASM and other adjacent structures.	Adenohypophyseal Diseases,Hypophyseal Disorders,Neurohypophyseal Diseases,Anterior Pituitary Diseases,Pituitary Disorders,Pituitary Gland Diseases,Posterior Pituitary Diseases,Adenohypophyseal Disease,Anterior Pituitary Disease,Disease, Adenohypophyseal,Disease, Anterior Pituitary,Disease, Neurohypophyseal,Disease, Pituitary,Disease, Pituitary Gland,Disease, Posterior Pituitary,Diseases, Adenohypophyseal,Diseases, Anterior Pituitary,Diseases, Neurohypophyseal,Diseases, Pituitary,Diseases, Pituitary Gland,Diseases, Posterior Pituitary,Disorder, Hypophyseal,Disorder, Pituitary,Disorders, Hypophyseal,Disorders, Pituitary,Hypophyseal Disorder,Neurohypophyseal Disease,Pituitary Disease,Pituitary Disease, Anterior,Pituitary Disease, Posterior,Pituitary Diseases, Anterior,Pituitary Diseases, Posterior,Pituitary Disorder,Pituitary Gland Disease,Posterior Pituitary Disease
D011196	Potassium Permanganate	Permanganic acid (HMnO4), potassium salt. A highly oxidative, water-soluble compound with purple crystals, and a sweet taste. (From McGraw-Hill Dictionary of Scientific and Technical Information, 4th ed)	Permanganate, Potassium
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006863	Hydrogen-Ion Concentration	The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH	pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D000303	Adrenal Cortex Diseases	Pathological processes of the ADRENAL CORTEX.	Adrenal Cortex Disease,Cortex Disease, Adrenal,Cortex Diseases, Adrenal,Disease, Adrenal Cortex,Diseases, Adrenal Cortex
D000440	Butanols	Isomeric forms and derivatives of butanol (C4H9OH).	Alcohols, Butyl,Butanol,Butylhydroxides,Hydroxybutanes,Butyl Alcohols
D013053	Spectrophotometry	The art or process of comparing photometrically the relative intensities of the light in different parts of the spectrum.
D015065	17-Hydroxycorticosteroids	A group of hydroxycorticosteroids bearing a hydroxy group at the 17-position. Urinary excretion of these compounds is used as an index of adrenal function. They are used systemically in the free alcohol form, but with esterification of the hydroxy groups, topical effectiveness is increased.	17 Hydroxycorticosteroids